Preparation of Biosurfactants using Waste from Oil Processes industry and Their Application

Abstract

Biosurfactants are microorganism-generated surface-active metabolites. In the 1960s, active research began on biosurfactants, which gained momentum in successive decades. More and more attention has been paid to biosurfactants as a result of their biodegradability, low harmfulness, monetary adequacy and capacity to produce inexhaustible and modest products. Locating a less expensive method of production is necessary to prove that biosurfactants can successfully replace synthetic ones. The presence of the carbon source and physical compound boundaries like air circulation, temperature and pH are the fundamental interaction properties that influence the structure and amounts of biosurfactant prepared. newlineComposition and emulsifying behavior of biosurfactants are dependent on both the producer strain and developmental stages. In this way, the existence of a carbon source, a nitrogen source, and the ratio of C:N, as well as dietary restrictions and synthetic and actual boundaries such as temperature, air circulation, divalent cations, and pH, affect the yield and type of polymer product. newlineThe main focus of this thesis is to prepare the rhamnolipid biosurfactant based on Pseudomonas aeruginosa MTCC 424 and rice bran residual oil for applications like enhanced oil recovery and deinking. Initially, procured Pseudomonas aeruginosa MTCC 424 culture was maintained on nutrient agar slants at 4°C for 24 hours in incubator shaker. Further, the cultivation of seed culture followed by the bacterial broth process in salt medium supplemented with 4% residual rice bran oil (RRBO) and 6% residual soybean oil (RSBO) in respective flasks as sole carbon source have been successfully processed for this comparative study. This thesis also includes the kinetic study of the prepared biosurfactant. newlineA brief layout of the thesis is as follows; the general overview about the recent scenario of surfactant, biosurfactant, factors influencing biosurfactant synthesis and biosurfactant preparation utilizing agro-industrial wastes are discussed in the