Chemical study of andrographis paniculata for pharmaceutical potentials

Abstract

Andrographis paniculata is an herbal medicine which is widely used in traditional system of newlinemedicine especially in Ayurveda for the treatment of many diseases in Asian countries Recently newlinesome new biological activities of Andrographis paniculata such as cytokine induction or newlinededuction T cell activation suppression a potential cancer therapeutic agent and its activity newlineagainst chronic and infectious diseases have been reported However the pharmaceutical potential newlineof Andrographis paniculata extracts has not been determined The main aim of the study was to newlinestudy and characterize the major active chemical present in the different parts of Andrographis newlinepaniculata Burm f Nees Family Acanthaceae for pharmaceutical potential To fulfill the aim newlineof the study fractionation of different parts using different solvents detailed study of Physicochemical newlineproperties physiological variation and study of the phytochemical profile of newlineAndrographis paniculata was carried out Anti oxidant activity and antimicrobial activity of newlinedifferent extracts was also analyzed w w content of the active component Andrographolide for newlinepharmaceutical potential was determined in different parts at different stages of life cycle and in newlinedifferent locations by HPTLC and HPLC analysis newlineVarious solvents acetone chloroform ethanol hexane methanol petroleum ether and water newlinewere used to prepare extracts of different samples and for fractionation Maximum yield of crude newlineof leaves and whole plant material drug of different samples was obtained in methanolic extracts newlinefollowed by the aqueous extracts Study of Physico chemical properties reveals that melting point newlineof whole plant material drug and leaves was 228 5oC 26oC respectively Results of extractive newlinevalue determination show that alcohol soluble extractive value was maximum and Pet ether newlinesoluble extractive value was least Among all samples studied alcohol soluble extractive value of newlineLL Leaves sample was highest 23 42 Results of physiological variation clearly indicate