Phytochemical and Pharmacological Investigation of some Indigenous Plants

Abstract

Diabetes mellitus is a group of metabolic disorders characterized by hyperglycemia, distorted metabolism of carbohydrates, lipids and proteins. The medicinal plants give a successful and safe therapy that is free of side effects because the treatment is lifelong.Thus, the current study aims towards the Phytochemical and Pharmacological investigation of some indigenous plants Bougainvillea glabrachoisy whole plant (Nyctaginaceae), Musa paradisiacaflower (Musaceae), Petrocarpusmarsupiumroxb. heartwood (Fabaceae) and Syzygiumcuminilinn.seeds(Myrtaceae) for treatment of Diabetes. newline The dried Bougainvillea glabrawhole plant (2Kg) was chopped into small pieces, re-dried, coarsely powdered, defatted with petroleum ether (60- 80and#730;C) and then extracted with ethanol (95%) in Soxhlet apparatus for 72 hours. The extract was then concentrated under reduced pressure to afford 240g (12%) dark blackish green mass. The fresh flower of M. paradisiaca(1.5kg) was chopped in small pieces. Chopped flower was successively macerated with ethanol by cold maceration for 7 days. The extract was filtered and concentrated under reduced pressure to give 84g (5.6%) of dark radish-brown mass. newline P. marsupium heartwood (2 Kg) and Syzygiumcumini(2 Kg) are extracted with ethanol (95%) in Soxhlet apparatus for 72 hours separately. 210g and 175 g dark greenish black mass obtained. newline The Phytochemical investigation involved isolation characterization and prediction of biological activities of the phytoconstituents obtend from ethanolic extract of the whole plant of Bougainvillea glabrachoisy. The extract was then concentrated under reduced pressure to afford 240 g (12%) dark blackish green mass. newline For column chromatography, the 95 percent ethanolic extract (120g) was chosen. The column was packed with silica gel particles ranging in size from 60 to 120 mesh. The ethanolic extract was eluted using a gradient of CHCl3: CH3OH in various ratios (in order of increasing polarity) to yield five fractions, A to F, with yields of 1.0g, 3.5g, 5.0g, 4.2g, 9.0g,