effect of dietary synbiotics on physio biochemical parameters and gene expression profile in broiler chicken

Abstract

Kapil Dev Prof. (Dr.) Rajiv Kant newlinePID No: 17PHBT204 (Advisor) newlineAbstract newline newlineUse of antibiotics as growth promoters (AGPs) in poultry production has lead to development of antibiotic resistance genes causing threats to humans and animals. Hence, antibiotic-free rearing of chickens has become increasingly popular due to increased awareness among consumers and their demand for healthy and safe chicken meat. Therefore, superior alternatives to AGPs are needed to maintain the production and gut health of chickens. The aim of the present study was to investigate the effect of dietary synbiotics [(prebiotic: Mannan-oligosaccharides (MOS) and probiotics: Lactobacillus acidophilus (LBA) and Bifidobacterium bifidum (BFD)] supplementation on physio-biochemical dynamics, productivity index, gut health, immune response, lipid metabolism and health indices, and gene expression profile in different tissues of broiler chicken. The study on the gene expression profile in different ranges of tissues was aimed to have an understanding of the mechanisms driving the physiology of broiler chicken growth, immunity, and lipid metabolism. In this study, the birds supplemented with 0.2% MOS along with LBA or BFD at 106 or 107 cfu/g feed showed better growth and health. From an economic point of view, synbiotic containing 0.2% MSO along with either LBA or BFD at 106 cfu/g feed was better compared to synbiotic containing 0.2% MOS along with either LBA or BFD at 107 cfu/g feed. Results also revealed that the supplementation of broiler chicken diets with synbiotic (0.2% MOS along with either LBA or BFD at 106 cfu/g feed) improved productivity index, lipid profile, and health indices via modulation of gene expression profile in the spleen, jejunum, and liver tissues. Birds fed 0.2% MOS along with LBA or BFD at 106 cfu/g feed had better meat yield and quality, and improved body antioxidant defence system with significant inhibition of lipid peroxidation. The study also revealed that the broiler chicken diets supplemented with 0.2%