Development and Characterization of Core Shell Nanoparticles for the Treatment of Alzheimer s Disease Using Non Invasive Approach

Abstract

newline newlineAlzheimer s disease is a type of neurodegenerative disease involving memory loss and loss of patients cognitive ability. At the end of 2017, there were 121,404 deaths from AD patients were recorded officially, and AD emerged as 6th prominent death cause in US and 5th prominent death cause in patients with age above 65. Americans with the population of 5.8 million recently are living with AD in 2019. However by the end of 2050, 14 million people were projected to be affected. Moreover, the drugs approved by USFDA exhibits Acetylcholinestarase inhibitors as pharmacological effect and these drugs too have limitations to reach brain due to its physicochemical properties and biological barriers to reach brain. In addition, flavonoids and carotenoids are another group of natural drugs of origin that showed antioxidant activity and attracted much attention from researchers due to its multipurpose nature and possible pharmacological action. As aforementioned these drugs also come across biological barriers through which they are unable to reach brain. Thus to overcome these barriers, it was hypothesized to develop core shell nanoparticles which can efficiently load both lutein and curcumin. Additionally, intranasal route was preferred to overcome the blood brain barrier by directly transferring drugs from nose to brain. newlineAim and Objectives: newlineAim was to develop and characterize core shell nanoparticles for the treatment of Alzheimer s disease using intranasal route. So the objectives of the investigation was to develop lutein and curcumin loaded core shell nanoparticles for increasing targeting efficiency to brain and was also compared with individual core nanoparticles. newlineMaterials and Methods newlineIn the present investigation, several types of nanoparticles were developed viz. chitosan nanoparticles, PLGA nanoparticles and chitosan coated PLGA core shell nanoparticles loaded with lutein and curcumin individually and including their respe