Evaluation of Radioprotective Potential of Selected Indian Bamboo leaves

Abstract

Radioprotector is the prime antidote to radiation injury. The burning urge for an ideal newlineradioprotector led us to explore natural resources. Medicinal plants, enriched with newlinephytochemicals having antioxidant properties can serve as a radioprotector. Bamboo species newlineis known for its antioxidant, anti-inflammatory, anti-cancer, anti-diabetic, cardioprotective, newlineneuroprotective, hepatoprotective and immunomodulatory activities and enriched polyphenolic newlinephytoconstituents spectrum. Thus it was selected for the present study. The study aims to newlineevaluate the protective effect of bamboo leaf extract against gamma radiation. Four bamboo newlinespecies Bambusa arundinacea (B.A), Bambusa vulgaris (B.V), Phyllostachys parvifolia (P.P), newlineand Dendrocalamus strictus (D.S) are selected for the study. The polyphenol rich leaf extract newlinewas prepared using 30% ethanol and standardized using eight polyphenols (chlorogenic acid, newlineorientin, isoorientin, vitexin, isovitexin, ferulic acid, luteolin, and apigenin) as biomarkers. The newlinestandardized extract was subjected to in vitro and in vivo screening of radioprotection. newlineQuantification of these selected polyphenols using RP-HPLC revealed that all the selected newlinepolyphenol were in the highest amount in B.A leaf extract. The order for the prevalence of the newlinebiomarkers in the selected species is as follow B.AgtD.SgtP.PgtB.V. The free radical scavenging newlineassays indicated the antioxidant potential of extract when compared to ascorbic acid. In vitro newlineprotective role of leaf extract was studied using cytokinesis blocked micronuclei assay (CBMN) newlinein human peripheral blood lymphocytes (HPBLs). CBMN results indicate that the treatment of newlineHPBLs with varying concentration of extract before exposure to different dose of gamma newlineradiation (4Gy and 6Gy) resulted in significant (plt 0.0001) decline of radiation induced newlinemicronuclei. It showed dose dependent and concentration driven activity with maximum newlineprotection ~ 70% at 9 and#956;g/ml concentration. In vivo protective effect was studied usin