Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/380882
Title: Overproduction of Bioethanol From Cellulosic Wastes
Researcher: Ningthoujam, Reema
Guide(s): Dhingra, Harish Kumar
Keywords: Chemistry
Chemistry Applied
Physical Sciences
University: Mody University of Science and Technology
Completed Date: 2021
Abstract: One of the most attractive lignocellulosic feedstock for the production of newlinebioethanol is the rice straw because of its cheaper and most abundantly found in newlinenature. Adding to it, it has high cellulose content that can be hydrolyzed into sugars newlineupon fermentation. The presence of lignin in the raw material makes it inferior in the newlineprocess of conversion of rice straw to bioethanol. Thus, selection of a suitable newlinepretreatment method that is cost-effective and which takes less time needs to be newlinedeveloped. Also, enzymatic hydrolysis is an essential step for degrading rice straw newlineinto fermentable sugars. Currently, for industrial bioethanol production, reseachers newlineare depending on the commercial enzymes that are quite expensive. Development of newlineinexpensive enzymes that can improve the parameters of hydrolysis is quite newlinenecessary. Finally, the heart in bioethanol production is the fermentation and newlinemicroorganisms such as yeasts are able to produce bioethanol in industrial scale. For newlinethis purpose, isolation of potential yeasts strains for obtaining maximum bioethanol newlineyield is required. newlineHence, in the present piece of work, we have isolated yeast species and newlinebased on biochemical, morphological and molecular analysis, the potential isolates newlinewere identified as Saccharomyces cerevisiae and Hanseniaspora guilliermondii. The newlineoptimum conditions for bioethanol production were also investigated. The newlineincubation time of 72 h was found to be best for bioethanol production by the best newlineisolate and maintaining the temperature and pH at 30°C and 4.5 respectively was newlinefound optimum. Physical treatment by grinding and milling followed by 4% NaOH newlinealkali treatment was found to be the best pretreatment technique and was newlineinvestigated by the FTIR, XRD and SEM analysis. Another fungus isolates which newlinewas identified as Aspergillus flavus was checked for its hydrolysis efficiency and newlinewas found the ability to produce cellulose enzyme. However, use of commercial newlineenzymes was found to be more efficient. The extracted bioethanol was analysed by newlinethe newline1 newlineH NMR and newline13 newlineC which confirms the presence of bioethanol. Strain newlineimprovement was done by mutagenesis which involves exposing the newlinemicroorganisms to UV rays and chemical mutagens such as EtBr and EMS. The newlinebioethanol yield of the wild strain (21.77%) was compared with that of the mutant newlinestrain (24.29%) which was achieved on treatment with EMS. Also, treatment with newlineEtBr also resulted in giving higher bioethanol yield but was found that not all the newlinemutants produced higher yields. Thus, searching for a favorable strain for improving newlinebioethanol production is in dire need. newline
Pagination: 
URI: http://hdl.handle.net/10603/380882
Appears in Departments:School of Liberal Arts and Sciences

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01_title.pdfAttached File367.79 kBAdobe PDFView/Open
02_declaration.pdf429.8 kBAdobe PDFView/Open
03_certificates.pdf951.44 kBAdobe PDFView/Open
04_acknowledgement.pdf447.24 kBAdobe PDFView/Open
05_contents.pdf401.04 kBAdobe PDFView/Open
06_list of graph and table.pdf293.69 kBAdobe PDFView/Open
07_abstract.pdf187.34 kBAdobe PDFView/Open
08_chapter 01.pdf548.15 kBAdobe PDFView/Open
09_chapter 02.pdf1.08 MBAdobe PDFView/Open
10_chapter 03.pdf902.17 kBAdobe PDFView/Open
11_chapter 04.pdf2.86 MBAdobe PDFView/Open
12_chapter 05.pdf467.91 kBAdobe PDFView/Open
13_chapter 06.pdf458.73 kBAdobe PDFView/Open
14_references.pdf751.8 kBAdobe PDFView/Open
16_publications.pdf24.5 MBAdobe PDFView/Open
80_recommendation.pdf24.86 MBAdobe PDFView/Open
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