Optimization of supercritical fluid extraction characterization and assessment of bioactivities of astaxanthin oil from processing discards of ridgeback shrimp Solenocera choprai Nataraj 1945

Abstract

This study envisaged the optimization of astaxanthin extraction using a greener extractiontechnique employing super critical CO2 (scCO2) and the evaluation of its in vitro and in vivo bioactivities. The Penaeid shrimp Solenocera choprai (Ridgeback shrimp), is widely distributed in the Indo-Pacific and is an important commercial shrimp species in India. The Industrial level processing of Solenocera choprai in Kerala and neighboring states has resulted in the accumulation of huge amount of processing discards. In this study, the identification of shrimp species was performed using the DNA barcode data of the mitochondrial gene cytochrome c oxidase subunit. Processing discards generated form newlinepeeled ridgeback shrimp was quantified as 59.7 %. Proximate analysis revealed that the newlineshrimp shell contained ample content of protein and fat that were rich in essential amino newlineacids, and and#969;-3 and and#969;-6 polyunsaturated fatty acids. Biorefinery concept was applied in newlineshrimp processing waste for extraction of biomolecules, viz. astaxanthin oil, protein, newlinechitin and chitosan by integrating different methods. Yield of astaxanthin in supercritical fluid extraction of astaxanthin oil was optimized using response surface methodology. The verification of the model was performed and the astaxanthin yield was 40.02 mg/kg.LC-MS/MS analysis quantified the concentration of astaxanthin in astaxanthin oil extracted. Astaxanthin oil showed strong antioxidant and antimicrobial activities. More importantly, it showed no cytotoxicity effect to cells which attributes its safety for further applications. Angiogenesis was increased with astaxanthin oil treatment, in a dose dependent manner. Cell migration assay revealed that the astaxanthin is capable to newlinemobilize the cells. Astaxanthin oil triggered elevation of catalase, SOD and GST in aged newlineanimals compared to control. The results of T-maze test indicated that the astaxanthin oil treated animals showed improved levels of learning and memory.