Investigation of the Anti inflammatory potential of Colebrookea oppositifolia by using in vitro and in vivo model

Abstract

Inflammatory diseases are becoming common in ageing societies throughout the world. newlineClinically used anti-inflammatory drugs suffer from the disadvantage of side effects and high newlinecost of treatment (in case of biologics). Traditional medicines and natural products offer a viable newlinealternative to these drugs. The present study was designed to investigate the protective effects of newlinelocally available traditional medicinal plants Ficus palmate, Waldheimia glabra, Zanthoxylum newlinearmatum and Colebrookea oppositifolia. Considering the traditional medicinal importance of newlinethese plants, both the ethanolic and hydroethanolic extracts of these plants were screened for newlinetheir anti-inflammatory potential. The results indicated that C. oppositifolia extracts displayed newlinemaximum anti-inflammatory potential both in-vivo and in-vitro as compared to other plants newlineunder study. Hence, C.oppositifolia was selected for further studies. The extracts were further newlinefractionated and pure compounds were isolated. Out of all the compounds, Hentriacontane, newlineKaempferol-3-O-and#946;-D-glucuronide (K3G) and 5,6,7- trihydroxyflavone-3-O-glucuronide methyl newlineester (TF3G) were found to be having maximum anti-inflammatory potential. Further insights newlineinto the mechanistic studies revealed that all these compounds showed the inhibition of proinflammatory cytokines TNF- and#945;,IL-6 and IL-1 and#946;. They also inhibited the pro-inflammatory newlinemediators like NO, PGE2 and LTB4. The results also established that the anti-inflammatory newlineactivity of these compounds is through the blockage of NF-kB pathway. newlineHence, the study establishes Hentriacontane, K3G and TF3G to be potential candidates for the newlineanti-inflammatory drug development industry. newline