Secondary metabolites production through in vitro regenerated plants of Lawsonia inermis L a multipurpose plant

Abstract

Lawsonia inermis L. belonging to family Lythraceae is popularly known as Henna or Mehendi. The plant is famous for its multipurpose uses in cosmetic, pharmaceutical and dyeing industries. Recently, the demand of raw plant material in these industries is increasing due to resurgence of the use of herbal products by people. Hence, immediate attention is required to scale up the production of this valuable multipurpose plant. Conventional propagation methods of stem cuttings and seeds in L. inermis are time consuming and also not preferred due to pest and disease problems. On the other hand, propagation through tissue culture techniques has potential for rapid multiplication of desired plant with similar genetic and biochemical make up within a short period of time. Rapid and efficient plant regeneration protocols and subsequent assessment of genetic and biochemical fidelity of the micropropagated plant is lacking in L. inermis. newlineAn efficient, reliable and reproducible plant regeneration protocol was developed for L. inermis. Multiple shoots were developed from mature nodal explants of Lawsonia inermis L. by axillary shoot proliferation. Murashige and Skoog s 1962 (MS) medium alone or supplemented with 6-Benzyladenine (BA; 0.25-4.0 mg L-1) or Kinetin (Kin; 0.25-4.0 mg L-1) or Thidiazuron (TDZ; 0.1-3.0 mg L-1) singularly was used for multiple shoot development. The nodal segments were further cultured on MS + 1.0 mg L-1 BA media supplemented with either Napthaleneacetic acid (NAA; 0.1-2.0 mg L-1) or Indole3-butyric acid (IBA; 0.1-2.0 mg L-1) or Gibberellic acid (GA3; 0.5-5.0 mg L-1) to check their influence on axillary shoot proliferation. Maximum number of shoot proliferation (81.6 %) with 7.8 shoots / explant was achieved on MS medium supplemented with 1.0 mg L-1 BA. Influence of seasonal variations and position of the mature nodes from shoot apex on axillary shoot proliferation was also carried out. It was observed that 3rd-5th nodes from shoot apex collected during the months of October-January were most su