Antioxidant and Anticancer Enhancing Potential of Various Fruit Extracts on Terminalia chebula

Abstract

Recently, a significant approach has been observed in the process of natural product medication newlineby the use of plants and plant based drugs. Terminalia chebula, a plant of deciduous forest newlinebelonging to the family, Combretaceae is believed to have a significant medicinal value due to its newlinetherapeutic potential. The present research was aimed at investigating the effect on antioxidant newlineand anticancer potential of Terminalia chebula when used in combination with a mixture of other newlinefruit extracts. The selected botanicals; Terminalia chebula, Punica granatum, Ananas comosus newlineand Phyllanthus emblica were extracted in aqueous, ethanol and aqueous-ethanolic (1:1) solvents newlineand 300 extract mixtures with different concentrations were formulated. The in-vitro antioxidant newlinescreening of 300 mixtures was carried out by hydroxyl radical (OH) scavenging method and 1,1- newlinediphenyl-2-picrlyhydrazyl (DPPH) assay. The screened mixtures were subjected to ex-vivo newlineantioxidant analysis by studying the inhibition of lipid peroxidation in packed cell volume, newlinecatalase and superoxide dismutase activity in erythrocyte lysate. The results of in-vitro newlineantioxidant assays revealed the ethanolic extract mixture to possess a higher antioxidant potential newlinewith an IC50 value of 11.2and#956;g/mL with an inhibition % of DPPH radical being 87.37±1.94 % at a newlineconcentration of 20and#956;g/mL as compared to the individual fruit extract of Terminalia chebula with newlinean IC50 value of 14.38and#956;g/mL and % inhibition 72.17±1.74 at 20and#956;g/mL. The results of enzymatic newlineex-vivo antioxidant analysis revealed the extract mixture to possess a better potency of 33.51% to newlinesave the degradation of superoxide dismutase as compared to individual fruit extract of T. newlinechebula with 44.56%. The qualitative phytochemical analysis of the extract mixture revealed the newlinepresence of multiple bioactive compounds. Additionally, anticancer potential of the extract newlinemixture was assessed against four cancer cell lines and the best results were obtained against the newlinebreast cancer cell line, MCF 7 with an IC50 value of 28.97