Phytochemical Characterization Antiproliferative and Apoptotic Efficacy of Green Synthesized Silver Nanoparticles Using Tabebuia roseo alba against Lung Cancer

Abstract

The present study was aimed at assessing the phytoconstituents present in Tabebuia roseo-alba followed by the determination of its anti-proliferative and apoptotic potential on lung cancer. The study was carried out in four phases. In phase I, the presence of phytochemical constituents in various solvent extracts of T. roseo-alba was screened by preliminary phytochemical screening, High performance thin layer chromatography. The results revealed the presence of various bioactive constituents like flavonoids,phenols,alkaloids,tannins and terpenoids in the leaves of T. roseo-alba. Further, based on the peaks obtained in GC-MS analysis, the presence of diverse group of pharmacologically active compounds were confirmed. To explore the therapeutic potential of the candidate plant free radical scavenging activity of various extracts of T. roseo-alba was carried out by following standard procedures. The results revealed the ability of ethanolic extract of T. roseo-alba in scavenging all the radicals tested in adose dependent manner. In phase II, silver nanoparticles of T. roseo-alba were synthesized using the ethanolic extract and they were characterized by adopting various parameters. In the present study, eco-friendly, cost effective green synthesis of silver nanoparticles using ethanolic extract of T. roseo-alba was explored and crystalline, spherical shaped, highly stable silver nanoparticles of varying size in the range between 5-100nm have been synthesized. In phase III, the anticancer activity of the ethanolic extract of T. roseo-alba and its AgNPs were determined by the MTT assay. It is evident from the results that, though both the samples were showing prominent antiproliferative activity on lung cancer cell lines, AgNPs of T. roseo-alba was found to be more potent than the ethanolic extract. Moreover, the ability to induce apoptosis was evaluated by FITC Annexin V and PI staining and the results have proved the efficiency of ethanolic extract of T. roseo-alba and its AgNPs to cause oxidative stress and subsequ