Phytochemical and Pharmacological Screening of Medicinal Plant for the Treatment of Hepatotoxicity

Abstract

Aim In the present study efforts were made to screen the phyto constituents obtained from two traditionally well known plant species namely T undulata and M hexandra for their hepatoprotective ability Method plant extract were subjected through a series of pharmacognostical study phytochemical study Invivo pharmacological activity has been performed in male albino wistar rats Bioassay guided isolation method was used in continous with column chromatography for identification of phyto components responsible for the activity Further Identified phyto constituents studied through insilico docking study Result Tests indicated the fact that the 70 percent ethanolic bark extract of T undulata and the ethyl acetate extract of M hexandra to be rich bioactive phytoconstituents Invivohepatoprotective study revealed that both our extracts namely 70 percent ethanolic bark extract of T undulata and the ethyl acetate extract of M hexandra showed very good treatment effect on the biochemical parameters and histological profiles Further column chromatography of extracts revealed the presence of a number of bioactive molecules which upon insilico screening by molecular docking on TACE protein divulged the fact that these molecules showed very good binding affinity with the said protein Conclusion It can thus be concluded that through the study we screened two species of plants namely T undulata and M hexandra for their hepatoprotective activity and scientifically validated their folkloric use We have tried to identify and list the probable phytoconstituents responsible for the hepatoprotective effect of our extract newline