Screening Phyto extracts from Marine and Terrestrial Sources for Anti Obesity Activity

Abstract

Aim In the present study efforts were made to screen the phyto constituents obtained from marine and terrestrial sources for antiobesity activity newlineMethod The Phyto or algal materials were collected and subjected to phytochemical screening Total phenolic content and total flavonoid content determination The Pharmacological screening methods involved invitro screening of constituents having pancreatic lipase inhibitory activity and in vivo method where in cafeteria diet induced obese rat models were used for the study newlineThe fractions obtained from the column were further subjected to spectral analysis and insillico screening via docking newlineResults Comparative analysis of Invitro and invivo screening proved the efficacy of the methanolic extract of Turbinaria ornata TOM marine alga better than all the other species which were analysed newlineThe Animals treated with Turbinaria ornata methanolic extract showed commendable antilipidemic activity the plasma biochemical parameters were also found to be significantly improved which was comparable to the control group Histological analysis also proved the efficacy of this extract Column fractionation helped in identifying a number of bioactive phyto molecules HPLC analysis identified presence of molecules such as Caffeic acid Gallic acid Chlorogenic acid Quercetin GCMS analysis identified a number of phytoconstituents which showed promising results in the insillico screening on two proteins responsible for obesity namely AMPK and HMGCOR newlineConclusion newlineWe conclude that the phyto constituets present in the marine brown alga Turbinaria ornata has potential to act as efficient anti obesity agent newline newline