Study on Biochemical changes and Molecular Basis of Polycystic Ovarian Syndrome PCOS in Selected Women and its Management with Glycyrrhiza glabra Root extract using Wistar Rat Model

Abstract

The present research work focused on Study on Biochemical changes and newlineMolecular Basis of Polycystic Ovarian Syndrome (PCOS) in Selected Women and its newlineManagement with Glycyrrhiza glabra Root Extract using Wistar Rat Model was carried newlineout in four phases. In the first phase, clinical features, risk factors and biochemical newlinechanges were analysed in normal, overweight and obese women with PCOS. The levels newlineof atherogenic lipids, glucose, insulin, reproductive hormones such as testosterone, LH, newlineFSH and estradiol were analysed. The level of C-reactive protein (CRP), thiobarbituric newlineacid reactive substances (TBARS) and the liver marker enzymes such as aspartate newlinetransaminase (AST), and alanine transaminase (ALT) activities were also measured in newlinePCOS women of both overweight and obese groups. The results showed that women in newlinethe age group of 19-32 were more affected by PCOS. Urban women were more affected newlineby PCOS than rural women. The risk factors for PCOS were found to be the family newlinehistory of PCOS, family history of diabetes mellitus, lack of physical exercise, obesity newlineand dietary habits. Biochemical findings suggested that abnormal lipid profile, insulin newlineresistance, elevated testosterone levels (hyperandrogenemia), low grade-inflammation newlineand abnormal functioning of liver play a major role in the pathogenesis of PCOS. To newlineunderstand molecular basis, in Phase II PCOS women were examined for INS VNTR and newlineFSHR gene polymorphisms to evaluate the association of these genes with PCOS. Since newlinegene sequence data were available for both these genes, PCR RFLP was chosen as the newlinetechnique to evaluate the role of these genetic factors in the manifestation of PCOS. We newlinehave found an association between the presence of Class III alleles and PCOS. Our study newlinealso proved the presence of T307A SNP may predispose south Indian women to PCOS. newlineIn Phase III, the different extracts of Glycyrrhiza glabra root were screened for the newlinepresence of phytoconstituents and antioxidant activities.