Efficacy of antigenic protein of streptococcus pyogenes in the treatment of wound infection in albino mice

Abstract

The current study aimed at the identification, isolation and purification newlineof the immunogenic protein of the Streptococcus. They are found to be the newlinemost prominent species in the diabetic wound ulcers that are becoming more newlinecommon in human beings. These are severe form of the infections caused by newlinethe Steptococcus pyogenes that are known to be fatal with most cases. Wide newlinenumbers of infection are caused by the S.pyogenes such as scarlet fever, newlineToxic Shock Syndrome (TSS), etc. Though there are several cases of wound newlineinfections, the most severe or fatal condition was known to be Necrotisiing newlinefasciitis The immunogenic protein was isolated from the bacteria and purified newlineby the basic biochemical techniques such as ion exchange and gel newlinepermeation chromatography, ultrafiltration, etc. The protein was further newlineanalyzed using polyacrylamide electrophoresis and characterized. The newlinepurified protein was known to be the M-protein which is the virulent protein newlinepresent on the surface of the S.pyogenes. The proteins play a greater role in newlinethe virulence and pathogenesis of the strain.There was a significant variation in the time period involved in the newlinewound healing. The wound which was treated with the protein healed well newlinewhen compared with the wound that was left untreated. There were other newlinegroups also whci were included in this study such as the wound with the swab newlineof Streptococcal culture, the wound with the culture as well as protein. There newlinewas a good improvement in the wound healing in the group of mice with newlinewound, bacterial culture and protein next to the wound with protein alone. newlineThere was a slow healing in the wound that had the bacterial culture swabbed newlineonto them. newline