Effectiveness of Celastrus paniculatus seed extract against lead acetate induced nephrotoxicity in Wistar rat

Abstract

The environmental pollutants such as heavy metals and harmful newlinechemicals alter the antaomy and physiology of the organ system. Lead acetate newlineis one of such pollutants, which is ubiquitously present in the environment. Kidney is the primarily targeted organ of lead toxicity because of its ability to newlinereabsorb and accumulate the lead. Many medicinal plants were found to newlinepossess interesting biological properties and hence the seeds of the plant newlineCelastrus paniculatus were condisered in this study for their nephroprotective newlineactivity by studying biochemical, oxidatant and antioxidant markers, histology newlineand immunohistochemistry. Electron microscope, protein expression, and gene newlineexpression were used to unequivocally confirm the activites studied. newlineIn conclusion, the study evaluated the efficacy of ethanolic extract of newlineCelastrus paniculatus seed against lead acetate induced nephrotoxicity, the newlineaspects covering biochemical, antioxidants, histology, immunohistochemistry, newlineScanning Electron Microscopy, and Western Blot (alpha GST expression) and newlineP13k/AKT pathway using RT - PCR were evaluated of the two doses of the newlineextract, the higher dose 800 mg/kg showed better protective effect in the animal newlinemodel. The order of protection was in the following order: Control gt EECPS 800 newlinegt EECPS 400 =NAC gt PbA. The study suggested that oral administration of newlinethe EECPS has ameliorated the changes in biochemical, oxidatant and newlineantioxidant parameters and other markers for renal damage. It also showed newlinerestoration of normal kidney tissue architecture in histological, newlineimmunohistochemical and scanning electron microscopic studies. newline