Daniellia Oliveri Rolfe hutch and dalziel leaves in type 2 diabetes treatment in Vivo in vitro and in silico approach

Abstract

Daniellia oliveri is a tree species belonging to the subfamily Caesalpinioideae (Fabaceae), whose young leaves are used locally to manage type 2 diabetes in Nigeria. newlineDiabetes mellitus is increasing in prevalence, and well-known traditional medications are associated with harmful effects. Hence, this study aimed to revalidate the use of newlineyoung leaves of D. oliveri and identified probable compounds responsible for the acclaimed activity. In streptozotocin-nicotinamide-induced type 2 diabetic rats, the newlinetherapeutic efficacy and haematological implications of administering organic extracts of Daniellia oliveri leaf (Do) were examined. Diabetes was induced in experimental rats newlinewith a single dosage of 60 mg/kg streptozotocin and 110 mg/kg nicotinamide and treated with 250mg/kg body weight extracts of D. oliveri obtained by cold maceration newlinein diethyl ether, ethanol, ethyl acetate, n-hexane, and 10 mg/kg metformin daily for 14 days. According to the findings of this investigation, Metformin and D. oliveri newlineextracts substantially lowered raised blood glucose levels and reversed increased glucose-6-phosphate dehydrogenase (G6PD) and glycosylated haemoglobin values. newlineHepatic glucose concentrations, white blood cells (WBC), packed cell volume (PCV), haemoglobin (Hb), and red blood cells (RBC) all rose in response to diabetes induction newlinebut decreased in response to D. oliveri extract administration. newlineMoreover, the inhibitory potentials of D. oliveri crude ethanolic extract (Do-C) and solvent-solvent fractions (n-hexane (Do-H), diethyl ether (Do-D), and ethyl acetate newline(Do-E)) obtained from Do-C on and#945;-amylase, and#945;-glucosidase activities in vitro and 1,1-diphenyl-2-picraylhydrazine (DPPH) radical scavenging activity were evaluated using newlinestandard protocols.All fractions inhibited DPPH free radicals effectively, with Do-E having excellent inhibition when compared with BHT. In this study, Do-C and its newlinesolvent-solvent fractions (Do-D, Do-E, and Do-H) inhibited and#945;-amylase and and#945;- glucosidase in a dose-dependent pattern.