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http://hdl.handle.net/10603/359849
Title: | Studies On Lens Culinaris B Galactosidase Purification Immobilization And Its Applications |
Researcher: | Yadav, Anjali |
Guide(s): | Kayastha, Arvind M |
Keywords: | Biotechnology and Applied Microbiology Galactose Lenses Life Sciences Microbiology |
University: | Banaras Hindu University |
Completed Date: | 2019 |
Abstract: | and#61656; and#946;-Galactosidase from Lens culinaris (Lsbgal) seeds were purified to apparent electrophoretic homogeneity. The enzyme was purified to 857 fold with a specific activity of 87 U/mg. newlineand#61656; Mr of Lsbgal under reducing and denaturing condition as determined from SDS-PAGE showed heterodimeric bands of 45 and 30 kDa, respectively. newlineand#61656; The Mr of native Lsbgal was calculated from Size-Exclusion Chromatography and was found to be 76 kDa. newlineand#61656; The optimum pH and temperature were found to be 3.0 and 58 and#8451;, respectively. newlineand#61656; ONPG was hydrolyzed at highest rate and Lsbgal showed a Km of 1.21 mM with it. newlineand#61656; Purified Lsbgal showed successful synthesis of Galacto-oligosaccharides (GOS) via transgalactosylation reaction when incubated with higher amount of lactose (200 g/L). newlineand#61656; Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) were carried out to analyze the products, which showed trisaccharides as a major products among total GOS. newline |
Pagination: | |
URI: | http://hdl.handle.net/10603/359849 |
Appears in Departments: | School of Biotechnology |
Files in This Item:
File | Description | Size | Format | |
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01_title.pdf | Attached File | 287.84 kB | Adobe PDF | View/Open |
02_certificate.pdf | 294.05 kB | Adobe PDF | View/Open | |
03_content.pdf | 118.06 kB | Adobe PDF | View/Open | |
04_abstract.pdf | 409.75 kB | Adobe PDF | View/Open | |
05_acknowledgement.pdf | 152.53 kB | Adobe PDF | View/Open | |
06_abbreviation.pdf | 117.72 kB | Adobe PDF | View/Open | |
07_chapter 1.pdf | 717.95 kB | Adobe PDF | View/Open | |
08_chapter 2.pdf | 779.17 kB | Adobe PDF | View/Open | |
09_chapter 3.pdf | 5.25 MB | Adobe PDF | View/Open | |
10_chapter 4.pdf | 1.75 MB | Adobe PDF | View/Open | |
11_chapter 5.pdf | 412.21 kB | Adobe PDF | View/Open | |
12_bibliography.pdf | 494.51 kB | Adobe PDF | View/Open | |
13_appendices.pdf | 13.85 MB | Adobe PDF | View/Open | |
80_recommendation.pdf | 700.22 kB | Adobe PDF | View/Open |
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