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http://hdl.handle.net/10603/359775
Title: | Development of Functionalized Iron Oxide Nanoparticles for Stem Cell Labeling and In Vivo Imaging |
Researcher: | Shelat Ruchita |
Guide(s): | Chandra Sudeshna |
Keywords: | Immunology Life Sciences |
University: | Narsee Monjee Institute of Management Studies |
Completed Date: | 2020 |
Abstract: | Osteochondral defects in the knee remain to be the most challenging clinical problem for orthopedic surgeons with maximum disability in young athletes and elder people. Owing to the restricted healing ability of articular cartilage, it is complicated to treat osteochondral defects leading to disabling pain and early osteoarthritis. Mesenchymal stem cells (MSCs) possess therapeutic potential for the treatment of osteochondral pain and pathology. The utilization of bone marrow derived MSCs (BM-MSCs) for cartilage regeneration is well reported due to self-renewal ability, immunomodulatory properties, easy propagation and differentiation into bone and cartilage. In spite of this, there is need of a reliable, clinically applicable cell tracker for deciphering the homing and distribution of stem cell post transplantation. newlineIn this study, we report the development of a stable and biocompatible iron oxide nanoparticle(IONPs) system that can be used for stem cell labelling as well as simultaneous in vivo monitoring using magnetic resonance imaging. Biocompatible magnetite (Fe3O4) iron oxide nanoparticles (IONPs) were synthesized using chemical co-precipitation method followed by surface modification with biomolecules like L-Arginine, L-Histidine, L-Lysine and and#946;-cyclodextrin. The characterization of synthesized IONPs were carried out based on the presence of functional group [Fourier transform infrared spectroscopy (FT-IR)], crystallite nature and size [x-ray diffraction (XRD)], particle size [transmission electron microscopy(TEM)], magnetization [vibrating sample magnetometry(VSM)] and surface charge [zeta potential]. All the characterization techniques validated the formation of crystalline superparamagnetic iron oxide nanoparticles for their use in vivo MR imaging. |
Pagination: | xxi;164 |
URI: | http://hdl.handle.net/10603/359775 |
Appears in Departments: | Department of Biological Sciences |
Files in This Item:
File | Description | Size | Format | |
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80_recommendation.pdf | Attached File | 225.9 kB | Adobe PDF | View/Open |
certificate.pdf | 177.12 kB | Adobe PDF | View/Open | |
chapter-1 introduction.pdf | 4.88 MB | Adobe PDF | View/Open | |
chapter-2 review of literature.pdf | 7.63 MB | Adobe PDF | View/Open | |
chapter-3 rationale, aim and objectives.pdf | 1.03 MB | Adobe PDF | View/Open | |
chapter-4 synthesis and characterization of ionps.pdf | 6.43 MB | Adobe PDF | View/Open | |
chapter-5 maintenance and characterization of bm-mscs.pdf | 6.76 MB | Adobe PDF | View/Open | |
chapter-6 cellular interaction studies of ionps and bm-mscs.pdf | 6.81 MB | Adobe PDF | View/Open | |
chapter-7 acute toxicity studies in rats.pdf | 3.37 MB | Adobe PDF | View/Open | |
chapter-8 optimization and development of osteochondral defect model in rats.pdf | 9.27 MB | Adobe PDF | View/Open | |
chapter-9 summary.pdf | 1.13 MB | Adobe PDF | View/Open | |
table of contents.pdf | 1.37 MB | Adobe PDF | View/Open | |
thesis first page.pdf | 64.92 kB | Adobe PDF | View/Open |
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