Effect of Caesalpinia Bonducella Against Nicotine Induced Nephrotoxicity in Wistar Rats A Molecular Study

Abstract

ABSTRCT newlineThe present study was aimed to assess the protective effect of Caesalpinia bonducella leaf extract on reactive oxygen species, antioxidant potential, and histopathological changes in nicotine-induced nephrotoxicity in male rats. After the treatment, control and experimental animals were subjected to reactive oxygen species (ROS), antioxidant enzymes, liver/kidney function markers, electrolytes, histopathological as well as nAChR TGF-and#946;, KIM-1, IL-18 and TNF-alpha mRNA and proteins expression. Nicotine-induced rats showed an increase in the levels of reactive oxygen species and LPO with concomitant alterations in the renal tissues. Conversely, enzymic and non-enzymic antioxidant levels were found to be decreased. However, an oral effective dose of C. bonducella leaf extract increased the antioxidant enzymes and retrieved the altered levels of ROS and LPO as well as histopathological changes which were induced by nicotine. C.bonducella increased the regucalcin and nAChR mRNA expression which were reduced in nephrotoxicity rats. TGF-beta, KIM-1, IL-18 and TNF-alpha mRNA expression were significantly reduced in C.bonducella treated nephrotixicity rats. Concurrently, significant diminution in KIM-1 and TGF-beta protein expression were observed in the kidney of nicotine-induced rats. Inflammatory signalling molecules such as TNF-alpha, IL-18 proteins were also significantly reversed to control level in C.bonducella treated toxicity rats.It is concluded from the present study that C.bonducella exhibit nephron-protective role through restoration of KIM1 and#945;-7nACR andinflammatory signalling molecules (IL-18 and TNF alpha) as well as serumbiochemical and histopathological in the kidney tissue of nicotine-inducednephrotoxicity in male rats. Hence, C.bonducella can be one of thepotential therapeutic drugs for the treatment of kidney diseases. This study proves the molecular mechanisms of action of C.bonducella on nicotine-induced renal toxicity. newline newline