Transcriptome Analysis of Host Pathogen Interaction of Adult Plant Resistance Gene in Wheat Leaf Rust

Abstract

Wheat (Triticum aestivum L) is the world s most widely cultivated food crop. It is affected by biotic and abiotic stresses, in which majority are biotic stresses. The biotic stress includes bacteria, fungi and viruses. Fungal diseases are economical important aspect in wheat production. Leaf rust (Lr) is the most common of the three wheat rust types. The gene Lr48 holds promise for durable resistance as APR. Thus to find out putative resistance genes s ESTs, we approached DDRT-PCR for the transcriptome analysis of host pathogen interaction of adult plant resistance gene in wheat leaf rust. newlineOut of 42 transcripts of DDRT-EST, 31 transcripts were successfully eluted. Out of 31 transcripts, 2 transcripts were differentially expressed at 0 hours post infection in inoculated sample and 0 in mock. 10 transcripts were differentially expressed at 24 hours post infection in inoculated sample and 4 in mock. 9 transcripts were differentially expressed at 48 hours post infection in inoculated sample and 1 in mock. 6 transcripts were differentially expressed at 72 hours post infection in inoculated sample and 1in mock. . 8 transcripts were differentially expressed at 168 hours post infection in inoculated sample and 0 in mock. newlineOut of 31, 29 transcripts were sequenced successfully in which 24 shows homology with related sequences. Submission of the 29 sequences of transcripts to the NCBI EST database was done. The homologous search by the tBLASTX in NCBI database significantly shows similarity with Triticum aestivum, Pucccinia spp. It also shows significantly similarity with abiotic and biotic stress related cDNA clones. newlineThe genetic information received from the DDRT EST will help in identification of genes/EST which are related to pathway of host pathogen interaction. The elucidation of gene information will be able to find out resistance genes which are helpful in identification of resistance varietal development of wheat leaf rust. Also it will be helpful in genomics of biotic stress tolerance. newline