Genetic improvement of linseed for inducing earliness in maturity and reducing the undesirable compounds through mutation

Abstract

newline The present investigation entitled, Genetic improvement of linseed for inducing newlineearliness in maturity and reducing the undesirable compounds through mutation was carried newlineout in the experimental farm of Department of Genetics and Plant Breeding, COA, CSK HP newlineKV, Palampur to assess the nature and magnitude of induced variability, determine the newlineinheritance pattern of earliness in induced lines; determine the relative proportion of oil newlinecontent of linseed mutants and isolate lines/mutants with reduced linolenic content in addition newlineto earliness. Experimental material comprised of three linseed varieties viz. Baner, Him Alsi2 and Surbhi, seeds of which were treated with five doses of gamma rays (20, 30, 40, 50, 60 newlinekR) and EMS (0.2 %, 0.4 %, 0.5 %, 0.6 %, 0.8 %) to generate M1 generation. On the basis of newlinesurvival percentage in M1 generation under lab conditions the LD50 dose was estimated i.e. newline96.63 kR (Baner), 72.94 kR (Him Alsi-2) and 53.22 kR (Surbhi) for gamma rays and 0.86 % newline(Baner), 0.72 % (Him Alsi- 2) and 0.35 % (Surbhi) for EMS. Plants harvested from M1 were newlinecarried forward to M2 generation. Both the mutagens were found effective and efficient in newlineproducing chlorophyll mutations and viable macromutations in M2 generation. Gamma rays newlineand EMS also found effective in producing variability in range and mean values for different newlinequantitative characters for which significant differences were obtained in both positive and newlinenegative directions. 113 mutants were carried forwarded to M3 generation based on their newlineperformance in M2 generation. Mean performance of M3 mutants showed significant shifts newlinefrom their respective control. Analysis of variance was found significant for all the characters newlinestudied. Low GCV and PCV was found for number of days to 75 per cent maturity but high newlineGCV (21.92 %) and PCV (27.05 %) was present in mutants for seed yield per plant. High newlineheritability (96.52) coupled with low GAM (7.00 %) was found for number of days to 75 per newlinecent maturity showing limited scope of its improvement, whi