Preparation of Strontium Substituted Bioceramic Scaffold Materials as a Potential Alternative for Bone grafts

Abstract

Tissue formation and repair is a complex cascade of events in which a number of growth newlinefactors are involved, controlled delivery of combinations of growth factors from scaffolds newlineappears to be a logical strategy in mimicking nature in applications such as tissue newlineengineering (TE). Now-a-days bone scaffolds are gaining importance with their porous newlinestructure, pore interconnectivity. Selection of suitable scaffold biomaterials for orthopaedic newlineapplications requires greater mechanical stability and tensile strength along with other basic newlinerequirements. Scaffold design method that would produce desired complex structure with newlineenhanced bioactivity is significantly challenging in tissue engineering approach. A variety of newlineceramic materials can be used as bone graft alternatives. Addition of biopolymers and newlinesubstitution of specific ions further enhance the bioactivity of scaffold and make it more newlinebiomimicking. newlineThe main objective of this thesis was to fabricate Strontium (Sr) substituted hydroxyapatite newline(HA) and biphasic calcium phosphate (BCP) scaffolds with adequate porosity and newlinecompressive strength by using polymeric sponge method. Sr substituted HA scaffolds were newlinefound to be porous biocompatible structures that demonstrared better proliferation for newlineosteoblast cells and also higher alakaline phosphatase (ALP) activity. BCP scaffolds were newlinefabricated with different proportions of Sr-HA and and#946; Tricalcium phosphate (and#946;-TCP) and the newlinevariation of physiochemical properties of the scaffolds were evaluated taking into newlineconsideration percentage of variation of the above two. In a study, biopolymers like guar newlinegum (GG) and gelatin were used as binder and biomimetic luffa sponge was used as a newlinetemplate for scaffold fabrication. Scaffolds synthesized with nano HA and Luffa cylinderica newline(LC) sponge obtained promising result in terms of bioactivity and imunocompatibility. The newlinefabricated scaffolds described here were evaluated for porosity, swelling ratio, newlinebiodegradability, lymphocyte and osteoblast spreading along with compressive strength. newlineFabri