Multiresidue Pesticide Analysis in Complex Food Matrices Using Novel Chromatographic Technoques an Analytical Approach

Abstract

The monitoring of residues of pesticides in the food is high priority objectives in newlinepesticide research world to get vigorous evaluation of the quality of food to avoid the newlinepossible risk caused to human health. For multiresidue analysis mass spectrometry is newlinea very selective and sensitive technology for detection and quantification of broad newlinerange of pesticides at very trace levels. For the analysis of residues of polar pesticides newlinein commodities of fruits and vegetables LC has emerged as very important technique newlineby choice and not fully explored. In addition to this, there is no straight forward newlinemethod for sample extraction and cleanup for detection of trace level residues of newlinepesticides. To avoid complications during the sample preparation, it is necessary to newlineadopt, validate the developed method. In view of this background information, the newlinepresent study was conducted to optimize the GC-MS/MS and UPLC instrument, newlinevalidation of the multiresidue method for the trace level detection of residues of newlinepesticides. A multi-residue method has been developed and validated for the newlinesimultaneous determination and quantification of 55 multiclass pesticides in fruit and newlinevegetable samples by GC-MS/MS with a triple quadrupole analyzer and 35 pesticides newlineby UPLC using UV detector. Samples were extracted by using QuEChERS (quick, newlineeasy, cheap, effective, rugged, and safe) extraction method with acetonitrile as newlinesolvent. An additional clean up step by using GCB (graphitized carbon black) and newlinePSA (primary secondary amine). Accuracy and precision were evaluated by means of newlinerecovery experiments in all the fruit and vegetable matrices spiked at two different newlineconcentration levels (for fruits 0.02 and 0.05mg/kg, for vegetables 0.01 and 01mg/kg). newlineRecoveries in most cases obtained between 70-110%. Matrix effects were tested by newlinecomparison of reference standards in pure solvent with matrix-matched standards. newlineFurther, this method was successfully employed for the analysis of real-world fruit newlineand vegetable samples. Analyzed results of fruit and vegetabe