Transcriptome Analysis of Oral Squamous Cell Carcinoma Using Next Generation Sequencing

Abstract

The importance placed on generating accurate NGS data, and newlineconsequently appropriate clinical interpretation, has stimulated much newlineinternational discussion regarding the creation and implementation of strict newlineguidelines and regulations for NGS clinical use. Compelling evidence supports newlinethe transition of next generation sequencing (NGS) technology from a research newlineenvironment into clinical practice. Before NGS technologies are fully adopted in newlinethe clinic, they should be thoroughly scrutinised for their potential as powerful newlinediagnostic and prognostic tools. In the context of clinical oncology, NGS newlinetechnologies are currently transitioning from a clinical research background into a newlinesetting where they will contribute significantly to individual patient cancer newlinemanagement. This study explores the steps that have been taken, and those still newlinerequired, for the transition of NGS into the clinical area, with particular emphasis newlineplaced on validation in the setting of clinical oncology. newlineThemolecularprofilesoforalcancer across the world populations vary and hence to newlineassess them is the existing priority to plan newlineappropriatetreatmentdespiterecentadvancements. newlineAmongavarietyofNextGeneration Sequencing(NGS)techniques,RNA newlinesequencingisassessmentofmRNA abundanceand transcriptsequence. newlineTodate,mostNGS-basedmetagenomicsanalyseshaveusedtostudythe required newlineoutput, NGS leads to a tremendous impact on the study of environmental and newlineclinical samples. In the relatively short timeframe since 2005, NGS has newlinefundamentally altered genomics research and allowed investigators to conduct newlineexperiments that were previously not technically feasibleoraffordable. newlineThisstudyaimstoexaminethegeneticandtranscriptionalvariation between the newlinecontrol and OSCC patients from Indian population using Next Generation newlineSequencing (NGS) to determine the oral cancer susceptibility. newline