Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/350409
Title: Molecular Characterization of PHB from Soil Bacterial Isolates
Researcher: Nausheen Sadaf Abdul Waris
Guide(s): Kamthane D. C.
Keywords: Life Sciences
Microbiology
University: Swami Ramanand Teerth Marathwada University
Completed Date: 2021
Abstract: PHB is a biopolymer which is produced as intracellular storage granules in most of the bacteria. These granules are having properties similar to properties like plastics, but they are completely biodegradable. This property of biodegradability makes them suitable as replacement to conventional plastics. Although numerous comprehensive studies have been performed on the production strategies of PHB, but most of them use chemically defined medium and genetically modified organisms for production. This causes a hike in production cost and in turn affects the final cost of product. The main reason behind unavailability of bioplastic for commercial use may be the production cost and use of genetically modified organisms for production. In this study, an attempt is taken to synthesize PHB by using bacterial isolates collected from natural habitats by using crude carbon sources like groundnut oil cake, paper pulp, whey, ficus racemosa, molasses, and used engine oil. After comparing the production efficiency, the efficient producers are subjected for 16s rRNA sequencing. The results confirmed that they were Bacillus megaterium (MZ 605042), Bacillus cereus (MZ 605040),, Pseudomonas aeruginosa(MZ 605041), Azotobacter chrococcum(MZ 605044) and Pseudomonas aeruginosa(MZ 605043). The produced PHB was also confirmed by using molecular techniques as FTIR, HPLC, GCMS, LCMS, DSC, 1H-NMR and 13C-NMR. All the graphs were confirmed by matching with that of standard PHB. The biodegradation studies of PHB were accomplished by using a liquid broth and a solid agar medium and it was observed that in both conditions the PHB is being utilized as carbon source by the microorganisms. newlineKeywords: PHB, bioplastic, 16s rRNA sequencing, FTIR, HPLC, GC-MS, LC-MS, DSC, 1H-NMR and 13C-NMR. newline
Pagination: 162p
URI: http://hdl.handle.net/10603/350409
Appears in Departments:Department of Microbiology

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02_certificate.pdf9.33 kBAdobe PDFView/Open
03_abstract.pdf9.93 kBAdobe PDFView/Open
04_declaration.pdf8.51 kBAdobe PDFView/Open
05_acknowledgement.pdf33.57 kBAdobe PDFView/Open
06_contents.pdf39.93 kBAdobe PDFView/Open
07_list_of_tables.pdf36.06 kBAdobe PDFView/Open
08_list_of_figures.pdf34.12 kBAdobe PDFView/Open
09_abbrevations.pdf28.61 kBAdobe PDFView/Open
10_chapter1.pdf376 kBAdobe PDFView/Open
11_chapter2.pdf120.72 kBAdobe PDFView/Open
12_chapter3.pdf95.81 kBAdobe PDFView/Open
13_chapter4.pdf635.4 kBAdobe PDFView/Open
14_chapter5.pdf72.62 kBAdobe PDFView/Open
15_conclusions.pdf48.46 kBAdobe PDFView/Open
16_bibliography.pdf184.01 kBAdobe PDFView/Open
80_recommendation.pdf91.23 kBAdobe PDFView/Open
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