Phytochemical Investigation And Anti Arthritic Studies On Some Indigenous Medicinal Plants

Abstract

The plants Alhagi pseudalhagi (Bieb.) Desv. (family: fabaceae) and Clerodendrum serratum (L.) Moon (family: lamiaceae) have considered traditionally for inflammation and gout, hence aerial part of Alhagi pseudalhagi (Bieb.) Desv and the leaf part of Clerodendrum serratum (L.) Moon were evaluated for phytochemical constituents and antiarthritic potential. newlinePhysicochemical evaluation of different parameters had been checked for both plants These reports would be of much significance in genuineness of the drug sample. Fluorescence analysis was performed on powder of Alhagi pseudalhagi (Bieb) Desv. and Clerodendrum serratum. The results were useful in the identification of authentic samples and recognizing adulterants. Powdered plant material of Alhagi pseudalhagi (Bieb.) Desv was extracted by soxhlet method at 600C with 95% v/v ethanol (APEE). Cold percolation method was used to obtain aqueous extract (APAE). The dried leaf part of Clerodendrum serratum (L.) Moon was extracted by hot percolation method at 550C using 95% v/v ethanol (CSEE). Aqueous extract was obtained by using cold percolation method (CSAE). APEE was fractionated by successive liquid-liquid partitioning method in 04 fractions with chloroform (APCF), ethyl acetate (APEA), methanolic (APMF) and aqueous (APAF). CSEE was subjected to fractionation in four fractions including hexane fraction (CSHF), chloroform fraction (CSCF), ethyl acetate fraction (CSEA) and methanolic fraction (CSMF). The entire fractions considered for TLC, HPTLC, FTIR, NMR, HRESI-MS analysis. The preliminary phytochemical screening was performed by using different reagents. Different compositions of mobile phase were tried using ternary mixtures of solvents including toluene, methanol, chloroform, ethyl acetate, acetic acid, formic acid, water. The extracts and fractions were applied on HPTLC plates using 100and#956;l syringe. The HPTLC fingerprinting analysis was considered using ethyl acetate: formic acid: acetic acid: water (10:1:1:1v/v/v/v) and toluene: ethyl acetate: formic acid (7:2:1