Chemopreventive Evaluation of an Indian Medicinal Plant on 1 2 Dimethyl Hydrazine Induced Aberrant Crypt Foci Rats Colon Cancer and Its Anti Oxidant Potential

Abstract

In India, nature has provided tremendous output of many herbal plants which possesses many pharmacological activities. In the present study we have taken an Indian medicinal plant (Portulaca quadrifida) which is used as vegetable in some places. As per epidemiological studies vegetables and fruits were used to cure many aliments as well as cancer. Therefore we have taken up the current study on one of the most common cancer that is colorectal cancer which has became more common in countries of low income both in men and women. Colon cancer which often found in people at the age of 50 or older. Usually colon cancer begins as polyps, if these polyps are screened and removed then colon cancer can be prevented. It is often a pathological outcome of continuous oxidative stress, governing to rupture of DNA, alterations in genes pertaining to cancer, further silencing epigenetically tumor suppressor genes leading to genomic instability in cellular excess production of reactive species of oxygen and nitrogen (ROS and RNS). ROS- induced carcinogenesis may be associated with oxidative DNA damage. Peroxidation of lipid is an ambiguity of aerobic life damaging wellness or wellbeing of man s present day life. Natural life systems which are lipid rich matrices are often open to auto oxidation if not saved from non enzymatic or endogenous enzymatic systems. In the present study Portulaca quadrifida was investigated for In vitro antioxidant studies in which aqueous extract has shown significant scavenging activity comparing with other extracts used and then plant was concealed for In vitro cytotoxicity discourse on colorectal cancer using cell lines like HT-29 and HCT-116 using MTT assay, By comparing all the successive extracts screened, aqueous extract showed highest inhibition with lowest concentration of 15.87 mg/ml. The in vitro data proved that among all the extracts AEPQ is having significant antioxidant and cytotoxicity effects hence; further in vivo studies were preceded with AEPQ. newline