Investigation on the production of microbial biodiesel using slaughterhouse wastewater as substrate

Abstract

Energy demand and depletion of fossil fuel source kept on increasing with continuous development and industrialization worldwide. This has resulted in the emission of harmful, noxious gases to the atmosphere. Most of the nation s development relies on fuel based economy and the current demand for energy makes fluctuation in petroleum products. This, in turn, increases the price of every product and activities of livelihood. To overcome this scenario, researchers are showing interest in a predominant, most affordable and eco-friendly alternate source for fossil fuels. As a result of its production of biofuel and its application gets focussed by most of the researcher. Based on the source of raw material, the biofuel evolved from first to second, second to third and third to the fourth generation. The limitations of every generation made way for the newer one. The third generation biofuel i.e. fuel derived from microorganisms has its own limitation like lack of feed source for microorganisms. There were several options to meet this limitation. Use of organic pollutant present in wastewater as a substrate for microorganisms is one such option and it was less concentrated so far. Most of the organisms lack the ability to grow and yield biodiesel feedstock when grown in wastewater medium. Identifying apt microorganism and growing in suitable wastewater medium results not only in producing alternate fuel feedstock but also it stabilizes the wastewater. This seems to be more concerned about the environment and economy. Enhanced studies on this option leading to commercialization make this solution a sustainable one. The organisms which have the ability to accumulate lipids for more than 20 % of their biomass were categorized as oleaginous microorganisms. They may be bacteria, yeast or algae. Many studies on algae newline