Studies on Nymphaea Pubescens Willd Nymphaeaceae a Plant Drug of aquatic Flora Interest

Abstract

The present study was aimed at establishing scientific validation with supporting data of the aquatic plant Nymphaea pubescens Willd family Nymphaeaceae on its pharmacognosy, phytochemistry and pharmacological activities. The study for the first time designed to focus on the pharmacognostic standardization, systematic isolation of the phytoconstituents, identification by spectroscopic interpretation including 2D NMR studies and subjecting the same for physico-chemical analysis like Lipinski rule of five, pharmacodynamic and pharmacokinetic parameters, screening the plant extracts and the isolated compounds for the target based antidiabetic activity in the type II diabetic animal model enzymatically and also at the receptor level, anti-cancer activity and In-vitro antioxidant activity in order to establish and standardize the folklore claims. The pharmacognostic standardization helps to differentiate between the species and adulterants or substitutes. The study revealed the macroscopic, microscopic identification, physico-chemical constants, powder and fluorescence analytical datas that provides the standardizing protocol for Nymphaea pubescens. Thoroughly investigated the literature, presence of macrocyclic flavonol Nympholide A and Nympholide B in Nymphaea lotus and Nuciferine in Nelumbo nucifera were isolated and reported. But so far no such compounds were isolated and scientifically proved from Nymphaea pubescens. We conclude that the aquatic plant Nymphaea pubescens is scientifically newlineproved by isolating the compounds 10-oxoundecanoic acid, 14-oxopentadec-9-enoic acid, Nuciferine and Quercetin and validating by physico-chemical property analysis, chemotaxonomical analysis, molecular studies of the extracts for the antidiabetic activity, molecular docking for the isolated compounds with the receptor Protein Tyrosine Phosphatase 1B, biological assay for active fraction in the DAL induced animal model and its free radical scavenging effect, since scientific validation is the hour of the day. newline