Validated Analytical Method Development for the Quantification of Multicomponent Drugs with Special Emphasis on Herbal and Synthetic Drug Formulations

Abstract

The present work involved the development of accurate, precise, simple and rapid Spectroscopic (UV) and Chromatographic (HPLC, HPTLC) methods for simultaneous estimation of the drugs in synthetic and herbal multicomponent formulations containing: Losartan potassium and Chlorthalidone, Acebrofylline and Acetylcysteine, Atovaquone and Proguanil, Lawsone and p-phenylenediamine, L- dopa in Zandopa herbal powder. This thesis invoves different chapter providing valuable information about the related topic studied detailed in this thesis. Chapter first deals with the brief introduction about modern analytical techniques like hplc, hptlc, and uv-spectroscopic method and gives brief information about the methods and techniques for the estimation of the multicomponent drug analysis. In this chapter also involves detailed about analytical method developments, analytical method validation, herbal analysis and forced degradation study. The Introduction of present work covers optimum information about the project name entitled here. The objectives of the study were to develop and validate methods for the estimation of drugs in multi components drug formulations by using HPLC, HPTLC, and UV Spectrophotometry was explained. In next chapter of this thesis deals with review of literature for the analytical methods available for the estimation of the selected drug formulation either present in single or in the combination with the other drug products. In fourth chapter discuss about the scope and planning for achieving the objectives of the study explain here. The material and methods chapter described with step by step procedure performed for the optimizing condition for the development of method on HPLC, HPTLC and UV Spectrophotometry methods and validation of the developed method were performed in accordance with ICH guideline and the methods also described the stability indicating assay method carried out by subjecting sample to the stress condition like acid, alkali, peroxide, neutral, thermal and UV light. newline newline