Elucidating the Anticancer Activity of Siddha Formulation Mahavallathy Leghiyam on 4 Nitroquinoline 1 Oxide Induced Oral Carcinogenesis In Rats

Abstract

Oral cancer is one of the major global threats to public health. They cause invasional metastasis by seeping into cell junctions, lymphatics till they reach major vascular tissues to establish into full-blown malignancy. Delayed progressive nature of this disease is a major challenge to inhibit it at the right time. Thus, to understand oral cancer 4NQO induced rat models as it mimics human pathological changes such as hyperplasia, dysplasia, severe dysplasia, carcinoma-in-situ and OSSC through molecular mechanisms similar to human neoplasm. Considering the relationship between oral cancer and impaired resistance function present field of research is moving towards therapeutic alternative indigenous medicines are capable of exhibiting anticancer effects against oral cancer in-vitro and in- vivo. Trypan blue exclusion dye test, MTT assay, PI, AO/Eb staining we carried out. After 28days dose fixation study, MVL chemo preventive effects were assessed. Dual stains revealed chromatin condensation, fragmentation and blebbing in in-vitro.In-vivo results revealed increased antioxidant activity, protein bound ATPases reached to normalcy and reduced MDA-DNA adducts and PCNA levels similar to that of positive control Quercetin. The current investigation showed MVL contains naturally occurring antioxidants that had the ability to induce apoptosis, possibly through caspase-3 intrinsic signaling pathway. Further, Maha Vallathi holds great promise for the use in chemo preventive and chemotherapeutic strategies. newline