Electrochemical DNA Biosensor for Detection of Dengue and Chikungunya Virus

Abstract

newlineDengue and chikungunya fevers have become a global health concern and the newlineconventional diagnostic assays employed for the detection of dengue and newlinechikungunya fever have major limitations. Thus, electrochemical DNA biosensors newlineserve as an effective alternatives. In view of the advantages of electrochemical DNA biosensors, the present investigation is aimed to develop DNA biosensors for the detection of dengue and chikungunya virus using various platforms. Herein, nanomaterials such as zinc oxide-platinum-palladium (ZnO/Pt-Pd) nanocomposites, graphene oxide-silicon dioxide (GO-SiO2) nanocomposites, molybdenum disulphide (MoS2) nanosheets, iron oxide-gold (Fe3O4atAu) nanocubes and zinc oxide (ZnO) tetrapods are being used as signal amplification interfaces, they also aid in effective immobilization of the DNA oligonucleotides. These nanomaterials are coated on various platforms or electrodes such as Fluorine doped tin oxide (FTO) electrodes, indium tin oxide electrode (ITO) etc. These electrodes served as platforms for nanoparticles and probe DNA immobilization. Herein, a newlinetransition from FTO to screen printed gold electrode (SPGE) to electrochemical paper newlineanalytical devices (ePADs) have been exploited. The FTO was used along with a newlinebulky three electrode set up containing Ag/AgCl as the reference electrode and newlineplatinum wire as the counter electrode. In order to simplify this set up, we switched to newlineSPGEs. These electrodes though were much simpler having all the three connections newlineon the same electrode but were very expensive. Thus, we finally switched to ePADs. newlineThese electrodes offer advantages such as these are economic and disposable and can newlinebe produced in mass easily. Thus, in the present study, we have developed five newlineelectrochemical DNA biosensors for the detection of dengue and chikungunya virus newlineusing different nanocomposites on FTO, SPGE and ePADs in order to obtain highly newlinespecific, sensitive, economic platform which has potential to convert into commercial newlinedevice. newlineChapter 1 newline newline