Investigation of alteration in insulin signaling due to prolonged hyperinsulinemia on 3T3 L1 adipocytes

Abstract

Type 2 diabetes mellitus T2DM is reaching epidemic proportions newlineworldwide with an increasing percentage of the adult population being affected newlineThe pathogenesis of T2DM results due to insulin resistance in target tissues with newlinean impairment in insulin secretion Among the various factors hyperinsulinemia newlineplays a crucial role in the development of insulin resistance Hyperinsulinemia newlinecondition develops into the two pathophysiological disorders T2DM and obesity newlineAlthough there are various pharmacological approaches for the management of newlineinsulin resistance they have been reported to exhibit several side effects As an newlinealternative to the existing allopathic treatments natural therapeutic approaches are newlinebeing investigated due to their limited side effects The present study investigates newlinethe role of hyperinsulinemia in impaired insulin signaling and explores an herbal newlinebased therapeutic approach for reverting hyperinsulinemia induced insulin newlineresistance in 3T3 L1 adipocytes newlineChronic hyperinsulinemia treatment in in vitro was studied by newlineincubating differentiated 3T3 L1 adipocytes with logarithmic 0 01 0 1 and 1 and#956;M newlinedoses of insulin referred to as hyperinsulinemia for different time periods 4 8 newlineand 12 h Hyperinsulinemia was found to impair insulin stimulated glucose newlineuptake in a dose and time dependent manner in 3T3 L1 adipocytes with a complete newlineinhibition observed at 12 h newline