New molecular insights in Biosynthesis translocation and Targeting of lipoproteins in bacteria For developing a versatile protein Engineering tool

Abstract

Anchoring hydrophilic proteins to hydrophobic membrane to newlineenable their functioning in the aqueous milieu when still anchored to the cell newlineis a useful adaptation in biological system In this regard, bacteria have newlineevolved a common way of modifying the N terminus of hydrophilic proteins newlineby attaching a lipid moiety N acyl S diacylglyceryl cysteine This newlinemodification also has potential applications in biotechnology especially in newlineELISA and biosensors where the hydrophilic proteins are required to anchor newlineand functionalize on hydrophobic surfaces like plastics However the newlineknowledge on bacterial lipid modification is limited especially in critical newlineaspects like translocation and targeting of lipoproteins A deeper newlineunderstanding on how bacterial lipoproteins are modified translocated and newlinetargeted is important while developing a versatile protein engineering tool newlinewith a generic design newlineExcept for the premise that amino acids at 2 position play a newlinecrucial role in lipoprotein targeting the exact signals that determine the final newlinelocalization remains unclear newline newline