Pharmacokinetic Studies of Some Selected Isolated Compounds of Herbal Drugs in various Dosage Forms

Abstract

The selected plants for the study such as Andrographis paniculata, Acorus Calamus, Phyllanthus amarus, Rauwolfia serpentina and Strychnous nuxvomica were extracted by maceration technique. Quantification analysis of compound separated by HPTLC can be carried by estimating the area of Band detection and results showed that 100mg of Andrographis paniculata, Acorus Calamus, Phyllanthus amarus, Rauwolfia serpentina and Strychnous nuxvomica, extract contains 4.563mg, 5.1mg, 6.17 mg, 1.16mg, 0.345mg, and 10.0 mg of pure Andrographolide, and#945;- Asarone, and#946;-Asarone, newlineHypophyllanthin, Reserpine and Strychnine respectively. The compatibility studies between the selected excipents and the pure drugs/extracts were studied. The IR spectra obtained were satisfactory with their characteristic absorption bands. Similarly the physical mixtures also indicated the presence of characteristic peak of the pure drugs / extracts and excipients. The formulated powders/granules were evaluated for their precompression parameters such as angle of repose, bulk density, tapped density, compressibility, drug content and their results were found to the satisfactory. Oral dosage forms such as Tablet, Spheroids, Pellets and Capsule were prepared with standard drug and herbal extract form. The dosage forms were evaluated for pre and post formulation parameters. Based on the results, best batches were taken for in vivo pharmacokinetic studies on rabbits. The pharmacokinetic data of Andrographolide, and#945;-and#946;-Asarone, Hypophyllanthin, Reserpine and Strychnine revealed the various parameters which are essential to project the exact dosage regimen of them in extract formulation, indicating the therapeutic activity of the herbal extract of Andrographis paniculata, Acorus Calamus, Phyllanthus amarus, Rauwolfia serpentina and Strychnous nuxvomica. It is suggested that based on the calculated pharmacokinetic data of the selected Herbal drugs should be formulated in various dosage forms for better dosage regimen and thereby expected to deliver the better therapeutic profiles.