Please use this identifier to cite or link to this item:
http://hdl.handle.net/10603/337157
Title: | Bio analytical method for the combination of Lipid lowering and Anti hypertensive agents |
Researcher: | Patel, Misari |
Guide(s): | Kothari, Charmy |
Keywords: | atorvastatin pharmacokinetic rosuvastatin |
University: | Nirma University |
Completed Date: | 2020 |
Abstract: | Even though the synergistic vascular protective effect of statins and angiotensin receptor blockers is quite known, the pharmacokinetic interaction among these two classes is yet to be understood facilitating the necessity of developing analytical methods for their determination. newlineHerein, a bio-analytical method using RP-HPLC/UV was developed and fully validated for simultaneous estimation of rosuvastatin and candesartan in human as well as rat plasma using atorvastatin as an internal standard. Solid-phase extraction was used for sample clean-up and its subsequent optimisation was carried out to achieve higher extraction efficiency and to eliminate matrix effect. A quality by design approach was used, wherein three-level factorial design was applied for optimisation of mobile phase composition and for assessing the effect of pH of the mobile phase using Design Expert Software. Adequate separation for both analytes was achieved with Waters C18 column (250 × 4.6 mm, 5 and#956;m) using ACN: 5 mM Sodium acetate buffer (70:30, v/v; pH adjusted to 3.5 with acetic acid) as a mobile phase at a flow rate of 1.0 ml/min and wavelength of 254 nm. The method when validated as per US-FDA guidelines was found to be precise as well as accurate. Extraction recovery observed for both analytes was above 85% as well as reproducible and consistent. Stability studies showed the samples to be stable over a long period covering from sample collection to final analysis. The validated method was successfully applied to Pharmacokinetic study in Wistar rats and the data did not reveal any evidence for a potential drug-drug interaction between ROS and CAN. This information provides evidence for clinical rational use of ROS and CAN. newlineSimilarly, a novel bio-analytical method using RP-HPLC/UV method was developed and fully validated for simultaneous estimation of pitavastatin and candesartan in human as well as rat plasma using telmisartan as an internal standard. The two analytes were extracted from plasma sample using liquid-l |
Pagination: | |
URI: | http://hdl.handle.net/10603/337157 |
Appears in Departments: | Institute of Pharmacy |
Files in This Item:
File | Description | Size | Format | |
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01_title.pdf | Attached File | 125.58 kB | Adobe PDF | View/Open |
02_certificate.pdf | 200.4 kB | Adobe PDF | View/Open | |
03_abstract.pdf | 278.93 kB | Adobe PDF | View/Open | |
04_declaration.pdf | 249.94 kB | Adobe PDF | View/Open | |
05_acknowledgement.pdf | 368.04 kB | Adobe PDF | View/Open | |
06_contents.pdf | 134.97 kB | Adobe PDF | View/Open | |
07_list_of_tables.pdf | 413.62 kB | Adobe PDF | View/Open | |
08_list_of_figures.pdf | 422.76 kB | Adobe PDF | View/Open | |
09_abbreviations.pdf | 292.49 kB | Adobe PDF | View/Open | |
10_chapter_1.pdf | 696.74 kB | Adobe PDF | View/Open | |
11_chapter_2.pdf | 674.64 kB | Adobe PDF | View/Open | |
12_chapter_3.pdf | 433.28 kB | Adobe PDF | View/Open | |
13_chapter_4.pdf | 870.75 kB | Adobe PDF | View/Open | |
14_chapter_5.pdf | 1.71 MB | Adobe PDF | View/Open | |
15_chapter_6.pdf | 1.38 MB | Adobe PDF | View/Open | |
16_chapter_7.pdf | 1.78 MB | Adobe PDF | View/Open | |
17_chapter_8.pdf | 1.56 MB | Adobe PDF | View/Open | |
18_chapter_9.pdf | 396.95 kB | Adobe PDF | View/Open | |
19_chapter_10.pdf | 516.64 kB | Adobe PDF | View/Open | |
20_chapter_11.pdf | 6.21 MB | Adobe PDF | View/Open | |
80_recommendation.pdf | 329.35 kB | Adobe PDF | View/Open |
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