Hepatoprotective mechanism of crinum asiaticum L and lycorine in carbon tetrachloride induced oxidative stress in swiss albino mice

Abstract

Oxidative stress is a major cause of several metabolic and genetic disorders. Natural antioxidants have an edge over synthetic ones with respect to safety concern, health benefits, potency and availability. Ever since the discovery of crude drugs from natural resources, there have been an urge to characterize and validate the bioactivity using modern techniques. The present investigation was designed to screen and validate the biopotency of the Crinum asiaticum (L) and lycorine in averting oxidative damage caused by carbon tetra chloride using Swiss albino mice as the model system. Two dimensional gel electrophoresis, MALDI-TOF and peptide mass fingerprint analyses revealed differential protein expression in the liver of the CCl4 induced, C.asiaticum and lycorine treated mice. HSP60, ATP synthase and Regucalcin have been identified, the up-regulation of which has significant role in maintaining the integrity of cellular proteins, ATP production, and calcium regulation. The hepatoprotective activity of Crinum asiaticum and lycorine was assessed from the assay of serum and liver pathological markers such as Aspartate Transaminase (AST), Alanine Transaminase (ALT), Lactate Dehydrogenase (LDH) and Alkaline Phosphatase (ALP) besides ascertaining the serum, hepatic and mitochondrial antioxidant status in control and experimental groups of mice. The contents of enzymatic and non-enzymatic antioxidants showed variations between the control and treatment groups. The extent of damage due to oxidative stress was evidenced from the banding patterns of matrix metalloproteinase and Lactate dehydrogenase.