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http://hdl.handle.net/10603/336487
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DC Field | Value | Language |
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dc.coverage.spatial | ||
dc.date.accessioned | 2021-08-17T09:59:36Z | - |
dc.date.available | 2021-08-17T09:59:36Z | - |
dc.identifier.uri | http://hdl.handle.net/10603/336487 | - |
dc.description.abstract | In context of the global threat of antimicrobial resistance (AMR) among pathogenic bacteria against conventional bactericidal antibiotics, investigation on complementary/ alternative approaches to manage bacterial infections is urgently warranted. Present study aimed at investigating the anti-pathogenic potential of Herboheal formulation (HF; a polyherbal formulation), and Phyllanthus emblica seed extract (PESE) against four different pathogenic bacteria viz. three antibiotic-resistant gram-negative bacteria- Chromobacterium violaceum, Serratia marcescens, Pseudomonas aeruginosa (latter two are #CDC-listed), and one gram-positive bacterium Staphylococcus aureus. This study has found both the extracts to be effective broad-spectrum quorum modulators, as they could affect production of QS (quorum sensing)-regulated pigments and other virulence traits in gram-positive as well as gram-negative bacterial pathogens. PESE and HF were found to exert in vitro quorum-modulatory effect on these pathogens at and#8805;50 and#956;g/mL and and#8805;0.025%v/v respectively. These extracts could curb the hemolytic activity of all the four test bacteria by 18-69%. Test formulations not only could inhibit (24-55%) biofilm formation, but also eradicated (18-63%) pre-formed biofilm effectively, and were also able to modulate bacterial antibiotic susceptibility and catalase activity (5-21%). Susceptibility of P. aeruginosa and S. aureus to lysis by human serum was enhanced under the influence of these extracts by 23-86%. Repeated exposure of both these notorious pathogens to PESE or HF did not induce resistance in them. In vivo assay demonstrated the ability of these extracts of attenuating (7-70%) bacterial virulence towards the model host C. elegans. Whole transcriptome analysis revealed certain genes associated with hemolysis, virulence, enzyme activity, transport, basic cellular processes (e.g. RNA metabolism), quorum sensing, and transcriptional regulators as the major targets of Herboheal in S. aureus. Both the extracts also showed notable p | |
dc.format.extent | ||
dc.language | English | |
dc.relation | ||
dc.rights | university | |
dc.title | Investigation on anti pathogenic potential of Herboheal formulation and Phyllanthus emblica L seed extract against selected human pathogenic bacteria | |
dc.title.alternative | ||
dc.creator.researcher | Patel, Pooja | |
dc.subject.keyword | bacterium | |
dc.subject.keyword | Biology and Biochemistry | |
dc.subject.keyword | Life Sciences | |
dc.subject.keyword | phytochemicals | |
dc.description.note | ||
dc.contributor.guide | Kothari, Vijay | |
dc.publisher.place | Ahmedabad | |
dc.publisher.university | Nirma University | |
dc.publisher.institution | Institute of Science | |
dc.date.registered | 2015 | |
dc.date.completed | 2020 | |
dc.date.awarded | 2021 | |
dc.format.dimensions | ||
dc.format.accompanyingmaterial | DVD | |
dc.source.university | University | |
dc.type.degree | Ph.D. | |
Appears in Departments: | Institute of Science |
Files in This Item:
File | Description | Size | Format | |
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01_title.pdf | Attached File | 323.67 kB | Adobe PDF | View/Open |
02_certificate.pdf | 62.83 kB | Adobe PDF | View/Open | |
03_ abstract.pdf | 257.71 kB | Adobe PDF | View/Open | |
04_declaration.pdf | 67.75 kB | Adobe PDF | View/Open | |
05_acknowledgement.pdf | 103.17 kB | Adobe PDF | View/Open | |
06_ contents.pdf | 206.56 kB | Adobe PDF | View/Open | |
07_list of tables.pdf | 259.26 kB | Adobe PDF | View/Open | |
08_ list of figures.pdf | 155.6 kB | Adobe PDF | View/Open | |
09_list of abbreviations.pdf | 364.86 kB | Adobe PDF | View/Open | |
10_chapter_1.pdf | 582.79 kB | Adobe PDF | View/Open | |
11_chapter_2.pdf | 1.19 MB | Adobe PDF | View/Open | |
12_chapter_3.pdf | 931.83 kB | Adobe PDF | View/Open | |
13_chapter_4.pdf | 4.36 MB | Adobe PDF | View/Open | |
14_conclusion.pdf | 274.58 kB | Adobe PDF | View/Open | |
15_ summary.pdf | 460.15 kB | Adobe PDF | View/Open | |
16_ references.pdf | 518.93 kB | Adobe PDF | View/Open | |
80_recommendation.pdf | 173.92 kB | Adobe PDF | View/Open |
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