Optimization and kinetics of bioethanol production from palm wood and banana peduncle wastes using kluveromyces marxianus

Abstract

Ethanol production was carried out using renewable resources of second generation lignocellulosic biomass. The fungal cells of Trichoderma reesei MTCC 4876, Kluveromyces marxianus MTCC 1389 were used for separate saccharification and fermentation, the substrates were palm wood and banana peduncle are separately pretreated with combination of different hydrolytic process like thermo-chemical methods of acid and following alkaline treatment then with ultra sonication to extract maximum carbohydrates. Fermentation was conducted with various process optimization conditions such as pH, temperature, rpm, substrate concentration, inoculums sizes using Response Surface Methodology, Artificial Neural Network and kinetics of biomass growth and product formation were also implemented to study the characteristics and behavior of reaction with process condition. The plant biomass palm wood and banana peduncle was pretreated using various ombination methods like 3% phosphoric acid, 3% nitric acid and 3% sulphuric acid with steam processing following ultra sonication treatment.. The hydrolysate of both the substrates was utilized for saccharification process after neutralizing and fermentation with Trichoderma reesei. The maximum glucose generated on palm wood using 3% sulphuric acid was 57.62 mg/g and 54.65 mg/g for banana peduncle with optimum condition of pH 4.5, temperature 30oC and agitator speed of 170 rpm. The cellulose enzyme activity released during the fermentation of saccharification was calibrated using Filter Paper Unit assay and found maximum on 3% sulphuric acid pretreated banana peduncle and palm wood wise 0.96 IU/ml and 1.0 IU/ml. newline