Development and validation of chemometrics assisted spectrophotometry and liquid chromatography for the simultaneous determination of some novel drugs in pharmaceuticals

Abstract

A simple and precise method involving four chemometric techniques, First order derivative and RP-HPLC was developed for the determination of various combinations of drugs in pharmaceutical formulations. The science of chemometrics can briefly be described as the interaction of certain mathematical and statistical methods to solve chemical problems. It has developed as a consequence of the change in the data obtained within chemistry with the emergence of new analytical techniques as well as microprocessors. Chemometrics has been developed by chemists using special calculation methods to solve chemical problems and has the unique ability that many of the methods in use have been developed from within the field itself. newlineThe object of this project is to develop: 1. Spectroscopic techniques in conjugation with chemometric tools. In order to achieve simple, rapid, precise and accurate analysis of combination of drugs in pharmaceutical formulation. Chromatographic separations were performed at ambient temperature with the use of respective mobile phase. The mobile phase was filtered and degassed before use. The flow rate of mobile phase was adjusted to 1.5 and 1 mL min-1. the detection wavelength was set at their nm. The injection volume of the standard and sample solutions was 20 and#956;Land#8722;1. The system suitability parameters as specified in USP results are found to be acceptable. The developed method was found to be accurate. Hence, good agreement was seen in the assay results of pharmaceutical formulation by chemometric, derivative spectroscopic and RP-HPLC. We conclude that the chemometric technique when coupled with CLS, MLR, PCR and PLS calibration methods sounds a good approach for obtaining reliable results, when compared with RP-HPLC method. Chemometric techniques are precise, rapid, selective, sensitive and less expensive. Hence it can be applied for the routine analysis of these combinations of drugs in the tablet formulation without any pre-treatment and without time consumption in quality control laboratories. newline