Study of anticancer nanoparticles for colorectal cancer

Abstract

In this study we have developed and optimized nanocarrier system for colorectal delivery using natural and colon specific polymers. The 5Fluorouracil and Irinotecan hydrochloride loaded polymeric nanoparticles were formulated and characterized for its physiochemical parameters. 5 Fluorouracil is potent anticancer drug used in therapy of colorectal cancer which is associated with the several limitations. So there is a need of alternate novel delivery system to enhance the drug availability in colon. To find out the physico chemical interaction between 5 Fluorouracil and Chitosan, FT-IR analysis were carried out and found to be compatible with each other. The crystallinity of the 5 Fluorouracil loaded chitosan nanoparticles was investigated using DSC and it was found that the drug included in the nanoparticles was in amorphous form. The statistical analysis was done for in vivo pharmacokinetic parameters. In conclusion, we developed a nanoparticulate drug delivery system for 5 Fluorouracil and Irinotecan hydrochloride using natural biodegradable polymer such as chitosan by ionic gelation method which is simpler, devoid of organic solvents and high temperature. The targeting efficiency was enhanced by conjugating with hyaluronic acid and the data obtained for their particle size, zeta potential, morphology, entrapment efficiency, drug loading, nanoparticle yield, in vitro drug release, in vitro cytotoxicity and its stability were found to be satisfactory. The preparation of 5 Fluorouracil and Irinotecan hydrochloride core tablets and their evaluation such as hardness, friability, disintegration, and uniformity content are within the I.P limits. Hence it may be recommended that the formulation of 5 Fluorouracil and Irinotecan hydrochloride as a nanoparticulate drug delivery system will be a promising drug release system for the therapy of colorectal cancer. The future studies may be carried out on clinical trials for the above formulations. newline