Development analytical standardization of anti diabetic herbal formulations and their activity evaluation by invitro methods

Abstract

Diabetes mellitus is a group of syndrome characterized by hyperglycemia resulting from defects in insulin secretion and insulin action or both. Great changes in the environment, behavior and lifestyle resulted in the raising rates of diabetes. It is more common among urban and rural Indians despite higher birth weight of urban babies. Tribal and rural population of India are highly dependent on the medicinal plants for meeting their health care needs. In the current study four anti-diabetic herbal drugs were chosen namely Cressa cretica, Terminalia catappa, Ficus glomerata and Vernonia anthelmintica to meet such needs. The proposed plan was to prepare extracts of selected plants, creating analytical profile of extracts by HPTLC and HPLC methods, evaluating the antidiabetic activity of the extracts and to prepare in-house formulations based on the in-vitro anti-diabetic activity of extracts. The medicinal plants were procured from different regions of Tamil Nadu and authenticated. All the four plants were extracted with petroleum ether, chloroform, ethyl acetate and methanol by hot continuous extraction method. Percentage yield of all the extracts were calculated where it was found to be maximum in the alcoholic extracts of all the medicinal herbs in the study. The methanolic extract of Cressa cretica yielded highest percentage with value of 10.5.The plant raw materials procured were analysed for its physiochemical properties which could be useful in quality control steps. Total Ash value, acid insoluble and water soluble ash values were determined. Water soluble extractives and alcohol soluble extractives were also determined. Physiochemical parameters were estabilished which could be useful in the quality control of selected plant species in the curent study. Composition of different phytochemical constituents present in all the extracts were found.