Please use this identifier to cite or link to this item: http://hdl.handle.net/10603/330040
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dc.date.accessioned2021-07-02T05:29:23Z-
dc.date.available2021-07-02T05:29:23Z-
dc.identifier.urihttp://hdl.handle.net/10603/330040-
dc.description.abstractWhole cell microbes having phosphatase activity were identified for the preparation of biosensor using Pikovskaya media for bacterial culture and Beneck s media for algal culture from the rhizospheric soil. Identified microbial colonies were tested for their phosphatase solubilizing capacity. Microbes having maximum solubilizing capacity were cultured and re-cultured using BG-11 inorganic media and were starved with respect to phosphate by suspending the media in phosphate free BG-11 media, before being used for immobilization on to the electrode surface. Starved microbes were immobilized on different electrode surfaces using bovine serum albumin (BSA) and were cross-linked with glutaraldehyde vapours for 30 minutes. The immobilized electrode was characterized for maximum output current by changing its cell density and optimum working environment by variation in pH of the solution. Immobilized electrode was also characterized for its composition, permeability and viability of the microbes in the membrane matrix with various techniques like scanning electron micrograph (SEM), cyclic voltammetry (CV) and spectrofluoremetry. Whole cell based amperometric biosensor was fabricated using an identified phosphatase solubilizing unicellular microalgae Chlorella sp. as the biocatalyst. The microbial culture was entrapped in a polymeric membrane of BSA directly interfaced to the surface of a platinum electrode for the detection of bioavailable heavy metal ions like zinc, copper, cadmium, cobalt and nickel. . The fabricated biosensor had a life time of 7 days and was found sensitive to a lower concentration level of 10and#8722;9 M of nickel ions, 10and#8722;10 M of cadmium and cobalt ions, 10and#8722;11 M of copper and zinc ions. The electrode system was workable in a detection range of 10-12 M to 10-6 M. The amperometric biosensor responds to heavy metal ions with a relative selectivity in the order: Zn2+ gt Cu2+ gt Cd2+ gt Co2+ gt Ni2+.
dc.format.extent94p.
dc.languageEnglish
dc.relation
dc.rightsuniversity
dc.titleMicrobial Biosensors for Some Heavy Metal Ions
dc.title.alternative
dc.creator.researcherSingh, Jasminder
dc.subject.keywordAmperometric
dc.subject.keywordBiosensors
dc.subject.keywordUltra sesitive
dc.description.note
dc.contributor.guideMittal, Susheel
dc.publisher.placePatiala
dc.publisher.universityThapar Institute of Engineering and Technology
dc.publisher.institutionSchool of Chemistry and Biochemistry
dc.date.registered
dc.date.completed2014
dc.date.awarded
dc.format.dimensions
dc.format.accompanyingmaterialNone
dc.source.universityUniversity
dc.type.degreePh.D.
Appears in Departments:School of Chemistry and Biochemistry

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02_certificate.pdf914.87 kBAdobe PDFView/Open
03_candidates declaration.pdf916.32 kBAdobe PDFView/Open
04_acknowledgements.pdf1.15 MBAdobe PDFView/Open
05_list of contents.pdf115.15 kBAdobe PDFView/Open
06_list of figures.pdf111.96 kBAdobe PDFView/Open
07_list of tables.pdf97.38 kBAdobe PDFView/Open
08_abstract.pdf135.27 kBAdobe PDFView/Open
09_chapter 1.pdf238.71 kBAdobe PDFView/Open
10_chapter 2.pdf343.33 kBAdobe PDFView/Open
11_chapter 3.pdf176.25 kBAdobe PDFView/Open
12_chapter 4.pdf1.87 MBAdobe PDFView/Open
13_list of publications.pdf96.19 kBAdobe PDFView/Open
80_recommendation.pdf1.92 MBAdobe PDFView/Open


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