Phytochemical antioxidant and cytotoxic activity of alangium salvifolium flowers

Abstract

Liver cancer is the third leading cause of cancer-related mortality in the world. The newlinetreatment and prevention of liver cancer are major needs that can most likely be enhanced by newlinea better understanding of the molecular process in cancer and development of cancer. newlineAlangium Salvifolium Wang. having tremendous historical evidence of being used in newlinetreatment of various ailments. In the preceding research after successive extraction with newlinepetroleum ether, ethyl acetate and methanol; the crude powder of flowers of Alangium newlinesalvifolium Wang.showed the presence of carbohydrate, glycosides, alkaloids, flavonoids, newlinephenols, tannins, and saponins. The cumulative phenolic content of ethyl acetate extract was newline95.300 mg/gm in Alangium salvifolium Wang.and 131.60 mg/gm in methanol. The overall newlineflavonoid content of ethyl acetate extract from Alangium salvifolium Wang.was 152.80 newlinemg/gm and 264.200 mg/gm for methanol extract. Spectral analysis of fractions by IR NMR newlineand MASS Spectra confirm the existence of benzene, hydroxyl protons. Mass spectrum thus newlineshows that the compound may have a quercetin glycoside nucleus. Present study established newlinethe antioxidant potential of Alangium salvifolium Wang.by studying the efficacy in newlinepreventing the in-vitro free radical using DPPH and reducing power assay. The DPPH radical newlinescavenging activity of pet ether extracts increased with increasing the concentration. newlineIn the in-vitro study, a significant amount of activity against the liver cell line was newlinereported for the isolated Alangium salvifolium Wang. (ASI) fraction. In the in-vivo study newlinesignificant inhibition of tumor growth was detected. In our study we observe the dramatic newlineincrease of LPO and SOD, GSH and catalase in carcinogenic rats, which is reversed by newlinesample treatments. These results show that both extracts and isolated portions provide newlinesignificant protection against oxidative stress in laboratory animals. It is also observed that newlineIL-6 (interleukin 6) and TNF-and#945; (tumor necrosis factor and#945;) was greatly reduced in presence of extracts.