Extraction phytochemical screening and cytotoxicity evaluation of compounds isolated from buchanania lanzan leaf on breast cancer cell line

Abstract

The most frequent cancer type in females in the world is breast cancer; with a lifetime risk of the order of 1/10. The tumor is malignant (cancer) if the cells can grow into (invade) surrounding tissues or spread (metastasize) to distant areas of the body. Treatment of breast cancer depends on the stage of the disease. Lines of treatment include mainly surgical removal of the tumor followed by radiotherapy or chemotherapy. Second lines including immunotherapy and other therapy and alternative medicine may represent a hope for breast cancer patients newlineBuchanania lanzan is a deciduous tree which produces seeds commonly known as chironji.The present study is based on the antioxidant and anti-cancerous potential of Buchanania lanzan which is also named as Chironji. Buchanania lanzan (chironji) is a tree species which belongs to the family Anacardiaceae and is commercially very useful. The plant extract has been studied for phytochemical analysis and spectral understanding in present science. In the present research after successive extraction with ethyl acetate and newlinemethanol; the crude powder of leaves of Buchanania lanzan showed the presence of carbohydrate, glycosides, alkaloids, flavonoids, phenols, tannins, and saponins. The complete phenolic compounds and flavonoids in quantities expressed as gallic acid equals and rutin equivalents were also quantitative study. The cumulative phenolic content of ethyl acetate extract was 19.33 in Buchanania lanzan and 32.66 in methanol. The overall newlineflavonoid content of ethyl acetate extract from Buchanania lanzan was 15.33 and 27.66 for methanol extract. The overall amount of phenolics and flavonoids in methanol extract was highest. Spectral analysis of fractions by IR and NMR Spectra confirm the existence of hydroxy and methylene protons. Mass spectrum thus shows that the compound may have a Celidoniol, Nonacosan-10-ol. The antioxidant activity of Buchanania lanzanin leaves was assessed based on scavenging activity of DPPH free radicals and Reducing power assay of different extract