Investigating the molecular mechanism governing reversal of amyloid beta induced memory loss in mice by transplantation of human umbilical cord blood derived lineage negative stem cells

Abstract

In the present study, we hypothesized that hUCB derived Lin-ve stem cells could reverse Amyloid and#946; induced memory loss augmented by enhanced neuroprotection. Our hypothesis proved correct as we could see the enhancement in the release of endogenous neurotropic factors after the transplantation of Lin-ve stem cells. We established memory loss in the mice using 1and#956;M concentration of Aand#946;42, validated by their behavior in the Morris water Maze. The loss in memory was found reversed through the transplantation of Lin-ve stem cells from the human Umbilical Cord Blood. The transplantation of stem cells gave following outcomes. First, Lin-ve stem cells rescue memory loss in Aand#946; induced memory loss by a possible involvement of astrocytic phagocytosis and clearance of the Aand#946;42 load in the demented brain.Second, transplantation of Lin-ve stem cells exert possible intra- hippocampal paracrine effects marked increase of neurotrophic factors. Third, stem cell transplantation exerts an anti-apoptotic response possibly mediated by the upregulated neurotropic factors. Fourth, Lin-ve stem cell rescues memory loss by the involvement of BDNF-TrkB pathway, which may initiate RET pathway as a result of their cross-talk. newline