Evaluation of anti diabetic activity from edible seeds

Abstract

This study aimed at evaluating the anti-hyperglycemic, lipase inhibitory properties of selected newline13 edible seeds (Nigella sativa, Momordica charantia, Benincasa hispida, Nelumbo nucifera, newlineCitrullus lanatus, Linum usitatissimum, Cucumis sativus, Cucurbita moschata, Trichosanthes newlinecucumerina, Luffa acutangula, Artocarpus heterophyllus, Salvia hispanica and Chenopodium newlinequinoa). Out of all evaluated methanolic and aqueous extract, 3 seeds (S.hispanica, C.quinoa newlineand N.nucifera) showed potent bioactivities. Therefore these three seeds were chosen for newlinefurther studies. Selected 3 seeds were combined to develop a polyherbal formulation (PF) newlineaccording to the combinations suggested by Response surface methodology (RSM) to find newlineout the best concentration of individual seeds in PF. RSM suggested that concentration at newlinewhich the three seed showed best activities was 1mg/ml of three seed in PF. Developed PF newlinewas evaluated for the presence of bioactive compound responsible for bioactivities of newlineindividual seed and PF using HPTLC. HPTLC results showed that rutin (a flavonoid) in newlineN.nucifera and PF was responsible for the therapeutic potential of PF. Developed PF was newlinecompared with commercially available anti-diabetic PF (Diabecon, Glucomap, BGR-34, newlineIME-9, Amree Plus) to get an idea about anti-diabetic and antioxidant potential of PF along newlinewith lipase inhibitory properties of developed PF. The results of comparative study indicated newlinethat PF was potential anti-diabetic agent exhibiting almost similar anti-diabetic activities in newlinecomparison of commercially available PF. Aq. and met. ext. of PF (125,250, 500 mg/kg of newlinebody weight) was evaluated for its anti-diabetic potential in diet induced obese (C57BL/6 newlinemale mice) model. PF met. ext. (500 mg/kg bw) showed better results in comparison of aq. newlineext. PF met. ext. was effective in improving tissue architecture of liver, kidney and pancreatic newlinetissue. As per our knowledge, this was the first attempt to study the anti-diabetic and newlineantioxidant potential of developed PF. The developed PF may be used as add on therapy for newlinediabetes, obesity and delaying or preventing diabetic secondary complications. newline