Efficacy of some ovulation synchronization protocols in dairy cows diagnosed with postpartum subclinical endometritis

Abstract

newline ABSTRACT newlineThe study was conducted on 45 post-partum dairy cows at Livestock Farm Complex, Dr. G.C. Negi College of Veterinary and Animal Sciences, CSK Himachal Pradesh Krishi Vishvavidyalaya, Palampur. Assessment of energy reserves i.e. body condition score (BCS) and back fat thickness (BFT), was done at weekly interval up to 8 weeks post-partum along with serum leptin estimation to investigate its association with occurrence of sub-clinical endometriris (SCE). For diagnostic purpose, endometrial cytology via cytotape method was carried out as confirmatory test for diagnosis of SCE i.e. polymorphonuclear cells (PMNCs) proportion and#8805;1 per cent. Also, trans-rectal Doppler ultrasonography at a weekly interval i.e. day 0 to 56, along with B-mode for assessment of uterine involution and resumption of ovarian activity, was used. Serum inflammatory markers such as Interleukin-6 (IL-6) and C-Reactive protein (C-RP) levels were estimated for drawing a relation with SCE. MG6G and MG6GP modified estrus synchronization protocols were applied to study their effect on PMNCs proportion during SCE and post-partum reproductive performance. newlineOn the basis of endometrial cytology, the incidence of sub-clinical endometritis positive and clinical endometritis positive cows was 60 and 8.89 per cent, respectively at 8 weeks post-partum, however, the cows in sub-clinical endometritis negative group had an incidence of 31.11 per cent. BCS and BFT were significantly lower (plt0.05) in SCEP cows as compared to SCEN cows on the day 0 and 56 post-partum, however, serum leptin concentrations were significantly low (plt0.05) only on day 0 post-partum in SCEP cows. Haemodynamic indices i.e. Time averaged maximum velocity and Blood flow volume- Time averaged maximum velocity, and diameter of middle uterine artery ipsilateral to gravid uterine horn was significantly higher (plt0.01 and plt0.05) on day 0 and 56 post-partum in SCEP and CEP cows as compared to SCEN cows. Serum concentrations of IL-6 and C-RP were significantly higher (plt0.01 and plt0.05) in SCEP and CEP cows as compared to SCEN cows at 8 weeks post-partum. MG6G and MG6GP modified estrus synchronization protocols led to a significant (plt0.05 and plt0.01, respectively) reduction in PMNCs proportion and serum IL-6 concentration on the day of estrus in cows under SCEP group. MG6GP modified estrus synchronization protocol fared better in terms of achieving conception i.e. 60 per cent vs. 30 per cent in MG6G protocol and also, resulted in a significant decrease (plt0.01) in days open of SCEP cows as compared to cows under SCEP control and CEP group. newline newline Student Major Advisor newline(Akshay Sharma) (Dr. Madhumeet Singh) newline newlineHead of the department newline